Abstract
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting are complementary methods for separating and detecting the presence of a specific protein from a complex mixture. Proteins, from a cell extract, for example, are separated electrophoretically through a polyacrylamide gel. Next, these are transferred onto a nitrocellulose membrane by electrical current, preserving the original banding pattern from the gel. The membrane is probed with an antibody specific for the protein of interest, forming an antibody-antigen complex that can be visualized by a variety of techniques. This chapter outlines a procedure for the analysis of a specific protein(s) from cultured, asexual stage Plasmodium falciparum by SDS-PAGE and Western blotting, using a minigel system. The entire procedure can be completed in one day. Most of the methods outlined below are applicable to proteins from a wide variety of sources.
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Cooper, R.A. (2002). SDS-PAGE and Western Blotting of Plasmodium falciparum Proteins. In: Doolan, D.L. (eds) Malaria Methods and Protocols. Methods in Molecular Medicine™, vol 72. Humana Press. https://doi.org/10.1385/1-59259-271-6:177
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DOI: https://doi.org/10.1385/1-59259-271-6:177
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