Abstract
This chapter provides standard methods for the incrimination of Anopheles mosquito species serving as malaria vectors and associated methods for measuring the intensity of transmission. In any malaria-endemic area, one or more species of Anopheles mosquitoes serve as malaria vectors. To show that an Anopheles mosquito species serves as a malaria vector in nature, it is necessary to demonstrate:
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1.
An association in time and space between the Anopheles species of mosquito and cases of malaria in humans. After study sites are selected, longitudinal field studies are established to sample mosquito populations. Adult mosquitoes are sampled by using trapping techniques such as landing/biting collections, light traps, pyrethrum spray catches inside houses, and outdoor aspiration collections. Larval mosquitoes developing in aquatic habitats normally are sampled by dipping methods. Mosquitoes are identified by standard taxonomic methods and also by molecular methods if mosquitoes belong to a species complex. The standard methods for performing landing/biting collections are described in this chapter; other types of mosquito trapping methods are described in refs. 1 and 3.
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2.
Evidence of direct contact between the Anopheles species and humans. Catching a mosquito biting humans through landing/biting catches conclusively establishes contact between that mosquito species and humans. A second method involves immunologically identifying human blood in the abdomen of field-captured Anopheles mosquitoes. A direct enzyme-linked immunosorbent assay (ELISA) suitable for bloodmeal identification of African malaria vectors is described in this chapter (3).
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3.
Evidence that the Anopheles species harbors malaria sporozoites in the salivary glands. Sporozoites may be detected in mosquitoes through the dissection and microscopic examination of mosquito salivary glands (4) or through ELISA methods (5). Both methods are described in this chapter.
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References
Service, M. W. (1976) Mosquito Ecology. Wiley, New York.
Service, M. (1993) Mosquito Ecology: Field Sampling Methods. Elsevier Applied Science, New York.
Beier J. C., Perkins, P. V., Wirtz, R. A., Koros, J., Diggs, D., Gargan, T. P., and Koech, D. K. (1988) Bloodmeal identification by direct enzyme-linked immunosorbent assay (ELISA), tested on Anopheles (Diptera: Culicidae) in Kenya. J. Med. Entomol. 25, 9–16.
WHO (1975) Manual on Practical Entomology in Malaria. Part II. Methods and Techniques. WHO Offset Publication 13, Geneva.
Wirtz, R. A. and Burkot, T. R. (1991) Detection of malaria parasites in mosquitoes. Chap 4 in Advances in Disease Vector Research, vol. 8, Springer-Verlag, New York, pp. 77–106.
Beier, J. C., Killeen, G. F., and Githure, J. I. (1999) Short report: Entomological inoculation rates and Plasmodium falciparum malaria prevalence in Africa. Am. J. Trop. Med. Hyg. 61, 109–113.
Beier, J. C., Copeland, R. S., Onyango, F. K., Asiago, C. M., Ramadhan, M., Koech, D. K., and Roberts, C. R. (1991) Plasmodium species identification by ELISA for sporozoites removed from dried dissection slides. J. Med. Entomol. 28, 533–536.
Kabiru, E. W., Mbogo, C. M., Muiruri, S. K., Ouma, J. H., Githure, J. I., and Beier, J. C. (1997) Sporozoite loads of naturally infected Anopheles in Kilifi District, Kenya. J. Am. Mosq. Control Assoc. 13, 259–262.
Beier, J. C., Onyango, F. K., Ramadhan, M., Koros, J. K., Asiago, C. M., Wirtz, R. A., et al. (1991) Quantitation of malaria sporozoites in the salivary glands of wild Afrotropical Anopheles. Med. Vet. Entomol. 5, 63–70.
Wirtz, R. A., Burkot, T., Graves, P. M., and Andre, R. G. (1987) Field evaluation of enzyme-linked immunosorbent assays for Plasmodium falciparum and Plasmodium vivax sporozoites in mosquitoes (Diptera: Culicidae) from Papua New Guinea. J. Med. Entomol. 24, 433–437.
Beier, J. C., Perkins, P. V., Wirtz, R. A., Whitmire, R. E., Mugambi, M., and Hockmeyer, W. T. (1987) Field evaluation of an enzyme-linked immunosorbent assay (ELISA) for Plasmodium falciparum sporozoite detection in anopheline mosquitoes from Kenya. Am. J. Trop. Med. Hyg. 36, 459–468.
Beier, J. C., Perkins, P. V., Koros, J. K., Onyango, F. K., Gargan, T. P., Wirtz, R. A., et al. (1990) Malaria sporozoite detection by dissection and ELISA to assess infectivity of Afrotropical Anopheles (Diptera: Culicidae). J. Med. Entomol. 27, 377–384.
Beier, J. C., Asiago, C. M., Onyango, F. K., and Koros, J. K. (1988) ELISA absorbance cut-off method affects malaria sporozoite rate determination in wild Afrotropical Anopheles. Med. Vet. Entomol. 2, 259–264.
Beier, J. C. and Koros, J. K. (1991) Visual assessment of sporozoite and bloodmeal ELISA samples in malaria field studies. J. Med. Entomol. 28, 805–808.
Edrissian, G. H., Manouchehry, A. V., and Hafizi, A. (1985) Application of an enzyme-linked immunosorbent assay (ELISA) for determination of the human blood index in anopheline mosquitoes collected in Iran. J. Am. Mosq. Control Assoc. 1, 349–352.
Burkot, T. R. and DeFoliart, G. R. (1982) Bloodmeal sources of Aedes triseriatus and Aedes vexans in a southern Wisconsin forest endemic for LaCrosse encephalitis virus. Am. J. Trop. Med. Hyg. 31, 376–381.
Beier, J. C., Oster, C. N., Onyango, F. K., Bales, J. D., Sherwood J. A., Perkins, P. V., et al. (1994) Plasmodium falciparum incidence relative to entomologic inoculation rates at a site proposed for testing malaria vaccines in western Kenya. Am. J. Trop. Med. Hyg. 50, 529–536.
Mbogo, C. N., Snow, R. W., Kabiru, E. W., Ouma, J. H., Githure, J. I., Marsh, K., and Beier, J. C. (1993) Low-level Plasmodium falciparum transmission and the incidence of severe malaria infec-tions on the Kenyan coast. Am. J. Trop. Med. Hyg. 49, 245–253.
Mbogo, C. N., Snow, R. W., Khamala, C. P., Kabiru, E. W., Ouma, J. H., Githure, J. I., et al. (1995) Relationships between Plasmodium falciparum transmission by vector populations and the incidence of severe disease at nine sites on the Kenyan coast. Am. J. Trop. Med. Hyg. 52, 201–206.
Mbogo, C. N., Glass, G. E., Forster, D., Kabiru, E. W., Githure, J. I., Ouma, J. H., and Beier, J. C. (1993) Evaluation of light traps for sampling anopheline mosquitoes in Kilifi, Kenya. J. Am. Mosq. Control Assoc. 9, 260–263.
Alles, H. K., Mendis, K. N., and Carter, R. (1998) Malaria mortality rates in South Asia and in Africa: implications for malaria control. Parasitol. Today 14, 369–375.
Garrett-Jones, C. and Shidrawi, G. R. (1969) Malaria vectorial capacity of a population of Anopheles gambiae. Bull. World Health Organ. 40, 531–545.
Dye, C. (1986) Vectorial capacity: must we measure all its components? Parasitol. Today 2 203–209.
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Beier, J.C. (2002). Vector Incrimination and Entomological Inoculation Rates. In: Doolan, D.L. (eds) Malaria Methods and Protocols. Methods in Molecular Medicine™, vol 72. Humana Press. https://doi.org/10.1385/1-59259-271-6:01
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DOI: https://doi.org/10.1385/1-59259-271-6:01
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