Abstract
A pioneering experiment in the early 1980s demonstrated that microinjection of recombinant growth hormone into the pronuclei of fertilized one-celled mouse embryos resulted in inheritable changes in the growth of these mice (1). Mammalian transgenic experiments have since contributed tremendously to our understanding of numerous complex biological processes. The power of the technique lies in that it allows the function, and developmental and physiological regulation of almost any protein to be studied within the context of the normal processes occurring in the whole animal.
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Si-Hoe, S.L., Murphy, D. (1999). Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs. In: Sharpe, P.T., Mason, I. (eds) Molecular Embryology. Methods in Molecular Biology™, vol 97. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-270-8:61
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DOI: https://doi.org/10.1385/1-59259-270-8:61
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-387-0
Online ISBN: 978-1-59259-270-8
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