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Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian

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Book cover Molecular Embryology

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 97))

Abstract

Our objective is to describe and critique some of the experimental embryological preparations used to analyze tissue interactions involved in neural induction in amphibians. The molecular basis of neural induction and the tissue interactions that carry the inductive signals are areas of active research, stimulated by the recent identification of several potential neural inducers (1–6), availability of regional molecular markers easily visualized with a good whole-mount RNA in situ hybridization method (7), and the work on Hox genes that may have a role in specifying regional differentiation of the vertebrate nervous system (8). These advances demand more of and make more useful the classical embryological manipulations used to characterize the tissue interactions involved in neural induction.

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Authors and Affiliations

  1. Department of Biology, University of Virginia, Charlottsville, VA

    Ray Keller & Ann Poznanski

  2. Graduate Group in Biophysics, University of California, Berkeley, CA

    Tamira Elul

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  1. Ray Keller
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  1. Dental and Medical Schools of Guy’s, King’s, and St. Thomas’s Hospitals, King’s College, London, UK

    Paul T. Sharpe  & Ivor Mason  & 

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Keller, R., Poznanski, A., Elul, T. (1999). Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian. In: Sharpe, P.T., Mason, I. (eds) Molecular Embryology. Methods in Molecular Biology™, vol 97. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-270-8:351

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  • DOI: https://doi.org/10.1385/1-59259-270-8:351

  • Publisher Name: Humana Press, Totowa, NJ

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