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In Situ Hybridization to Sections (Nonradioactive)

  • Maria Rex
  • Paul J. Scotting
Part of the Methods in Molecular Biology™ book series (MIMB, volume 97)

Abstract

In situ hybridization (ISH) takes advantage of the ability of mRNA within a cell to hybridize with exogenously applied complementary RNA (riboprobes) or DNA molecules. This interaction is visualized by labeling the applied nucleic acid probe with a detectable molecule (radioactive, such as 35S, or non-radioactive, such as digoxygenin [DIG]). The technique allows patterns of gene expression to be visualized in many tissues or cell types simultaneously.

Keywords

Color Detection Sheep Serum Blue Tetrazolium Chloride Alkaline Phosphatase Streptavidin Fume Cupboard 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Maria Rex
    • 1
  • Paul J. Scotting
    • 1
  1. 1.Department of GeneticsUniversity of NotinghamUK

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