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Grafting Hensen’s Node

  • Claudio D. Stern
Part of the Methods in Molecular Biology™ book series (MIMB, volume 97)

Abstract

Soon after Spemann and Mangold’s (1) famous demonstration in 1924 that the dorsal lip of the blastopore of the gastrulating amphibian embryo has the unique ability to induce a second axis when grafted into an ectopic site in a host embryo, Waddington (2,3) showed that Hensen’s node is its equivalent in amniotes. After transplanting this region into an ectopic site in interspecific combinations of rabbit, duck, and chick embryos, he found that a second axis developed, where the nervous system was derived from the host ectoderm (4). Hensen’s node is situated at the anterior (cranial) tip of the primitive streak during gastrulation, and in chick embryos appears as a bulbous thickening, some 100 μm in diameter, centered around a depression, the primitive pit. At this point, the three germ layers of the embryo are in very close apposition.

Keywords

Chick Embryo Pasteur Pipet Primitive Streak Watch Glass Vitelline Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Spemann, H. and Mangold, H. (1924) Über Induktion von Embryonanlagen durch Implantation artfremder Organisatoren. Wilh. Roux Arch. EntwMech. Organ. 100, 599–638.Google Scholar
  2. 2.
    Waddington, C. H. (1932) Experiments on the development of chick and duck embryos, cultivated in vitro. Phil. Trans. R. Soc. Lond. B 221, 179–230.CrossRefGoogle Scholar
  3. 3.
    Waddington, C. H. (1933) Induction by the primitive streak and its derivatives in the chick. J. Exp. Biol. 10, 38–46.Google Scholar
  4. 4.
    Streit, A., Théry, C., and Stern, C. D. (1994) Of mice and frogs. Trends Genet. 10, 181–183.PubMedCrossRefGoogle Scholar
  5. 5.
    Hamburger, V. and Hamilton, H. L. (1951) A series of normal stages in the development of the chick embryo. J. Morph. 88, 49–92.CrossRefGoogle Scholar
  6. 6.
    New, D. A. T. (1955) A new technique for the cultivation of the chick embryo in vitro. J. Embryol. Exp. Morph. 3, 326–331.Google Scholar
  7. 7.
    Selleck, M. A. J. and Stern, C. D. (1991) Fate mapping and cell lineage analysis of Hensen’s node in the chick embryo. Development 112, 615–626.PubMedGoogle Scholar
  8. 8.
    Storey, K. G., Selleck, M. A. J., and Stern, C. D. (1995) Induction by different subpopulations of cells in Hensen’s node. Development 121, 417–428PubMedGoogle Scholar
  9. 9.
    Storey, K. G., Crossley, J. M., De Robertis, E. M., Norris, W. E., and Stern, C. D. (1992) Neural induction and regionalisation in the chick embryo. Development 114, 729–741.PubMedGoogle Scholar
  10. 10.
    Stern, C. D. (1994) The avian embryo: a powerful model system for studying neural induction. FASEB J. 8, 687–691PubMedGoogle Scholar
  11. 11.
    Streit, A., Stern, C. D., Théry, C., Ireland, G. W., Aparicio, S., Sharpe, M., and Gherardi, E. (1995) A role for HGF/SF in neural induction and its expression in Hensen’s node during gastrulation. Development 121, 813–824PubMedGoogle Scholar
  12. 12.
    Spratt, N. T., Jr. (1947) A simple method for explanting and cultivating early chick embryos in vitro. Science 106, 452.PubMedCrossRefGoogle Scholar
  13. 13.
    Abercrombie, M. (1950) The effects of antero-posterior reversal of lengths of the primitive streak in the chick. Phil. Trans. Roy. Soc. Lond. 234, 317–338CrossRefGoogle Scholar
  14. 14.
    Stern, C. D. and Ireland, G. W. (1981) An integrated experimental study of endoderm formation in avian embryos. Anat. Embryol. 163, 245–263.PubMedCrossRefGoogle Scholar
  15. 15.
    Stern, C. D. (1993) Avian embryos, in: Essential Developmental Biology: A Practical Approach (Stern, C. D. and Holland, P. W. H., eds.), IRL at Oxford University Press, Oxford, UK, pp. 45–54.Google Scholar
  16. 16.
    Stern, C. D. (1993) Transplantation in avian embryos, in: Essential Developmental Biology: A Practical Approach. (Stern, C. D. and Holland, P. W. H., eds.), IRL at Oxford University Press, Oxford, UK, pp. 111–117.Google Scholar
  17. 17.
    Stern, C. D. and Holland, P. W. H. (eds.) (1993) Essential Developmental Biology: A Practical Approach. IRL at Oxford University Press, Oxford, UK.Google Scholar
  18. 18.
    Selleck, M. A. J. and Stern, C. D. (1992) Commitment of mesoderm cells in Hensen’s node of the chick embryo to notochord and somite. Development 114, 403–415.Google Scholar

Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Claudio D. Stern
    • 1
  1. 1.Department of Genetics and DevelopmentCollege of Physicians and Surgeons of Columbia UniversityNew York

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