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Flavoenzyme Structure and Function

Approaches Using Flavin Analogues

  • Protocol
Flavoprotein Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 131))

Abstract

Flavoenzymes are redox proteins that catalyze a wide diversity of biological reactions ranging from O2 activation, to aromatic hydroxylations, dehydrogenations, and reactions in which they can accept or donate one or two electrons. In addition, they can fulfill structural and regulatory roles. This diversity is due to the fine tuning of the reactivity of the isoalloxazine ring of the flavin coenzyme by specific interactions with the protein moiety on the particular enzyme it complexes with. Methods to provide specific information on these interactions have relied on three approaches:

  1. 1.

    Determination of the three-dimensional structure of the flavoenzyme by X-ray diffraction techniques.

  2. 2.

    Spectroscopic probes of the protein influence on flavin structure by techniques such as nuclear magnetic resonance (NMR), resonance Raman, fluorescence, electron paramagnetic resonance (EPR), and circular dichroism (CD) spectroscopies.

  3. 3.

    Replacement of the native flavin coenzyme with suitable flavin analogues designed to ask specific questions regarding the reactivity, the accessibility, and the mode of specific interactions with the protein.

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Author information

Authors and Affiliations

  1. Departments of Biochemistry and Chemistry, Emory University School of Medicine, Atlanta, GA

    Dale Edmondson & Sandro Ghisla

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  1. Dale Edmondson
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  2. Sandro Ghisla
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Editors and Affiliations

  1. University of Edinburgh, Edinburgh, UK

    Stephen K. Chapman  & Graeme A. Reid  & 

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© 1999 Humana Press Inc., Totowa, NJ

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Edmondson, D., Ghisla, S. (1999). Flavoenzyme Structure and Function. In: Chapman, S.K., Reid, G.A. (eds) Flavoprotein Protocols. Methods in Molecular Biology, vol 131. Humana Press. https://doi.org/10.1385/1-59259-266-X:157

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  • DOI: https://doi.org/10.1385/1-59259-266-X:157

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-734-2

  • Online ISBN: 978-1-59259-266-1

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