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Vibrational Spectroscopy of Flavoproteins

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Flavoprotein Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 131))

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Abstract

Several techniques under the broad umbrella of “vibrational spectroscopy” have aided in the identification of structural and electronic factors associated with the catalytic mechanisms of many biological systems (1). A complete elucidation of the mechanism of function of a biological center requires knowledge of the exact nature of bond strengths and their dependence upon the protein environment. By probing the intrinsic vibrational properties of species of interest, which are almost invariably prosthetic group(s), one can obtain information on interactions as diverse as molecular recognition events (2) and mechanistic intermediates (3) to dynamics associated with ligand/substrate binding (4) and protein motions (5). Flavin-containing proteins are a vital component of numerous enzyme systems, participating in a myriad of functions within organisms. Their roles range from drug detoxification to aromatic acid synthesis (6,7). Several spectroscopic techniques have been applied to studying the molecular properties of flavoproteins in order to understand more fully their functional basis as efficient electron donors/acceptors. In this chapter one shall deal exclusively with what vibrational spectroscopy has provided and can offer towards our understanding of the inter-relationships between molecular properties and flavoprotein function. A brief outline of theory and instrumentation is followed by an extensive experimental protocol for the associated techniques.

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Author information

Authors and Affiliations

  1. Department of Chemistry, University of Strathclyde, Glasgow, Scotland

    Iain D. G. Macdonald

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  1. Iain D. G. Macdonald
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Editor information

Editors and Affiliations

  1. University of Edinburgh, Edinburgh, UK

    Stephen K. Chapman  & Graeme A. Reid  & 

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© 1999 Humana Press Inc., Totowa, NJ

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Macdonald, I.D.G. (1999). Vibrational Spectroscopy of Flavoproteins. In: Chapman, S.K., Reid, G.A. (eds) Flavoprotein Protocols. Methods in Molecular Biology, vol 131. Humana Press. https://doi.org/10.1385/1-59259-266-X:125

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  • DOI: https://doi.org/10.1385/1-59259-266-X:125

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-734-2

  • Online ISBN: 978-1-59259-266-1

  • eBook Packages: Springer Protocols

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