Abstract
When a bimane-labeled peptide is bound to donor vesicles incorporating small amounts of a nonexchangeable energy-transfer acceptor such as DABS-PC (1) or NBD-PE, the fluorescence is strongly quenched. If a population of acceptor vesicles containing neither peptide nor quencher is subsequently added in large excess to the peptide-donor mixture, the redistribution of the peptide between the donor and acceptor populations can be monitored through the resultant time-dependent enhancement of fluorescence.
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References
Shahinian, S. and Silvius, J. R. (1995) Doubly lipid-modified protein sequences exhibit long-lived anchorage to lipid bilayer membranes. Biochemistry 34, 3813–3822.
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© 1998 Humana Press Inc.
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Silvius, J.R. (1998). Determination of the Kinetics of Intervesicle Transfer and Transbilayer Diffusion of Bimane-Labeled Lipidated Peptides. In: Gelb, M.H. (eds) Protein Lipidation Protocols. Methods in Molecular Biology, vol 116. Humana Press. https://doi.org/10.1385/1-59259-264-3:187
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DOI: https://doi.org/10.1385/1-59259-264-3:187
Publisher Name: Humana Press
Print ISBN: 978-0-89603-534-8
Online ISBN: 978-1-59259-264-7
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