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Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin

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Book cover Eicosanoid Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 120))

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Abstract

Immunocytochemical analysis is an effective method for providing a differential assessment of protein expression and localization in tissue samples. A variety of methods exist for immunocytochemical analysis including: immunofluorescence, immunoperoxidase, and streptavidin—biotin linked methods. Furthermore, both paraffin embedded and frozen tissue samples can be utilized for immunohistologic analysis.

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References

  1. DeLellis, R. A. (1981) Basic techniques of immunochemistry, in Diagnostic Immunohistochemistry (DeLellis, R. A., ed.), Masson Publishing, New York, pp. 7–16.

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  2. Elias, J. (1990) Immunohistopathology: A Pratical Approach to Diagnosis, ASCP, Chicago.

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  3. Buckman, S. Y., Gresham, A., Hale, P., Hruza, G., Anast, J., Masferrer, J., and Pentland, A. P. (1998) COX-2 expression is induced by UVB exposure in human skin: implications for the development of skin cancer. Carcinogenesis, in press.

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© 1999 Humana Press Inc.

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Buckman, S.Y., Koki, A.T., Edwards, D.A., Pentland, A.P. (1999). Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin. In: Lianos, E.A. (eds) Eicosanoid Protocols. Methods in Molecular Biology, vol 120. Humana Press. https://doi.org/10.1385/1-59259-263-5:35

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  • DOI: https://doi.org/10.1385/1-59259-263-5:35

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-667-3

  • Online ISBN: 978-1-59259-263-0

  • eBook Packages: Springer Protocols

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