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Rapid Purification of DNA Topoisomerase II Containing a Hexahistidine Tag by Metal Ion Affinity Chromatography

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Book cover DNA Topoisomerase Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 94))

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Abstract

Since DNA topoisomerases have become a major focus for scientists, the purification of these proteins from all sources represents one of the basic hurdles on the way to investigating this important class of enzymes. A variety of methods consisting of numerous steps have been applied to obtain a reasonable amount of pure enzyme (1-4).

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Author information

Authors and Affiliations

  1. Department of Hematology and Oncology, Medizinische Hochschule Hannover, Hannover, Germany

    Harald Biersack

  2. Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA

    Sanne Jensen

  3. Department of Molecular and Structural Biology, Aarhus University, Aarhus, Denmark

    Ole Westergaard

Authors
  1. Harald Biersack
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  2. Sanne Jensen
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  3. Ole Westergaard
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Editor information

Editors and Affiliations

  1. St. Jude Children’s Research Hospital, Memphis, TN, USA

    Mary-Ann Bjornsti

  2. Vanderbilt University School of Medicine, Nashville, TN, USA

    Neil Osheroff

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© 1999 Humana Press Inc.

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Biersack, H., Jensen, S., Westergaard, O. (1999). Rapid Purification of DNA Topoisomerase II Containing a Hexahistidine Tag by Metal Ion Affinity Chromatography. In: Bjornsti, MA., Osheroff, N. (eds) DNA Topoisomerase Protocols. Methods in Molecular Biology, vol 94. Humana, Totowa, NJ. https://doi.org/10.1385/1-59259-259-7:235

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  • DOI: https://doi.org/10.1385/1-59259-259-7:235

  • Published:

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-0-89603-444-0

  • Online ISBN: 978-1-59259-259-3

  • eBook Packages: Springer Protocols

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