Abstract
The mechanism of action of DNA topoisomerase I in catalyzing the relaxation of supercoiled DNA and how this reaction is perturbed by the antitumor drug camptothecin have been the subject of intense investigation (reviewedin (1-3). Much of this effort has focused on structure/function studies of wild-type and mutant forms of the enzyme, derived from a variety of sources (1,4-20). The budding yeast Saccharomyces cerevisiae has proven particularly amenable to the genetic manipulations required to overexpress and purify wild-type and mutant forms of eukaryotic DNA topoisomerase I (3,4,12,13).
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Bjornsti, MA., Fertala, J. (1999). Overexpression and Purification of DNA Topoisomerase I from Yeast. In: Bjornsti, MA., Osheroff, N. (eds) DNA Topoisomerase Protocols. Methods in Molecular Biology, vol 94. Humana, Totowa, NJ. https://doi.org/10.1385/1-59259-259-7:179
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DOI: https://doi.org/10.1385/1-59259-259-7:179
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