Purification and Use of DNA Minicircles with Different Linking Numbers

  • Giorgio Camilloni
  • Rodolfo Negri
  • Micaela Caserta
  • Ernesto Di Mauro
Part of the Methods in Molecular Biology™ book series (MIMB, volume 94)


The structural organization of both prokaryotic and eukaryotic chromosomes has evolved following a common principle: the need for storing the genetic information in topologically independent domains consisting of one or more genes and of all the elements required in cis for their functioning. The elements that belong to the same functional unit are topologically linked: they depend on each other and cooperate.


Xylene Cyanole Negative Supercoiling Versus Constant Voltage Minor Groove Width Weiss Unit 
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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Giorgio Camilloni
    • 1
  • Rodolfo Negri
    • 2
  • Micaela Caserta
    • 3
  • Ernesto Di Mauro
    • 1
  1. 1.Dipartimento di Genetica e Biologia MolecolareUniversitd di RomaRomaItaly
  2. 2.Centro di Studio per gli Acidi Nucleici (CNR), Dipartimento di Genetica e Biologia MolecolareUniversitá di RomaRomeItaly
  3. 3.Centro di Studio per gli Acidi Nucleici (CNR), Dipartimento di Genetica e Biologia MolecolareUniversitd di RomaRomeItaly

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