Abstract
Screening cDNA libraries by transient expression in mammalian cells has proven to be very effective for the isolation of cDNAs encoding secreted, surface, and intracellular proteins.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Lee, F., Yokota, T., Otsuka, T., Meyerson, P., Villaret, D., Coffman, R., Mosmann, T., Rennick, D., Roehm, N., Smith, C∼., Zlotnik, A., and Arai, K. (1986) Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T-cell and mast cell stimulating activities. Proc. Natl. Acad. Sci. USA 83, 2061–2065.
Yang, Y., Ciarletta, A. B., Temple, P. A., Chung, M. P., Kovacic, S., Witek-Giannotti, J. S., Leary, A. C., Kitz, R., Donahue, R. E., Wong, G. C., and Clark, S. C. (1996) Human IL3 (Multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3. Cell 47, 3–19.
Seed, B. and Aruffo, S., (1987) Molecular cloning of the human CD2 antigen by a rapid immunoselection procedure Proc. Natl. Acad. Sci. USA 84, 3365–3369.
Aruffo, S. and Seed, B., (1987) Molecular cloning of a CD28 cDNA by a high efficiency COS cell expression system. Proc. Natl. Acad. Sci. USA 84, 8573–8577.
Seed, B. (1987) An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its ligand CD2. Nature 329, 840–842.
Armitage, R. J., Fanslow, W. C., Strokbine, L., Sato, T. A., Clifford, K. N., Macduff, B. M., Anderson, D. M., Gimpel, S. D., Davis-Smith, T., Maliszewski, C. R., Clark, E. A., Smith, C. A., Grabstein, K. H., Cosman, D., and Spriggs, M. K. (1992). Molecular and biological characterization of a murine ligand for CD40. Nature 357, 80–83.
Suda, T., Takahashi, T., Golstein, P., and Nagata, S. (1993) Molecular cloning and expression of the Fas ligand, a novel member of the tumor necrosis factor family. Cell 75, 1169–1178.
Lyman, S. D., James, L., Vanden Bos, T., de Vries, P., Brasel, K., Gliniak, B., Hollingsworth, L. T., Picha, K. S., McKenna, H. J., Splett, R. R., Fletcher, F. A., Maraskovsky, E., Farrah, T., Foxworthe, D., Williams, D. E., and Beckmann, M. P. ( 1993) Molecular cloning of a ligand for the flt3/flk-2 tyrosine kinase receptor: a proliferative factor for primitive hematopoietic cells. Cell 75, 1157–1167.
Simmons, D., Makgoba, W., and Seed, B. (1988) ICAM-1, an adhesion ligand of LFA1, is homologous to the neural cell adhesion molecule NCAM. Nature 331, 624–627.
Fawcett, J., Holness, C. L., Needham, L., Turley, H., Gatter, K., Mason, D., and Simmons, D. (1992) Molecular cloning of ICAM-3, a third ligand for LFA-1, constitutively expressed on human leukocytes. Nature 360, 481–484.
Stamenkovic, I., Amiot, M., Pesando, J., and Seed, B., (1989). A lymphocyte molecule implicated in lymph node homing is a member of the cartilage link protein family Cell 56, 1057–1062.
Simmons, D., Walker, C., Power, C., and Pigot, R., (1990). Molecular cloning of CD31, a putative intercellular adhesion molecule closely related to carcinoembryonic antigen. J. Exp. Med. 171, 2147–2152.
Simmons, D. and Seed, B. (1988) Isolation of a cDNA encoding CD33, a differentiation antigen of myeloid progenitor cells. J. Immunol. 141, 2791–2800.
Simmons, D., Satherthwaite, A. B., Tenen, D. G., and Seed, B. (1992) Molecular cloning of a cDNA encoding CD34, a sialomucin of human hematopoietic stem cells. J. Immunol. 148, 267–271.
Osborn, L., Heission, C., Tizard, R., Vassallo, C., Luhowskyi, S., Chi-Rosso, G., and Lobb, R. (1989) Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytes. Cell 59, 1203–1211.
Staunton, D., Dustin, M., and Springer, T. (1989) Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1. Nature 339, 61–64.
Horst, E., Wijngaard, P. L. J., Metzelaar, M., Bast, E. J. E. B., and Clevers, H. C. (1991) A method for cDNA cloning in COS cells irrespective of the subcellular site of expression. Nucl. Acids Res. 19, 4556.
Metzelaar, M. Wijngaard, P. L. J. Peters, P. J., Sixma, J. J., Nienwenhuist, H. K., and Clevers, H. C. (1991) CD63 antigen, a novel lysosomal membrane glycopro-tein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. J. Biol. Chem. 266, 3239–3245.
Strathdee, C. A., Gavish, H., Shannon, W. R., and Buchwald, M. (1992) Cloning of cDNAs for Fanconi’s anaemia by functional complementation. Nature 356, 763–767.
Miyata, T., Takeda, J., Iida, Y., Yamada, N., Inoue, N., Takahashi, M., Maeda, K., Kitani, T., and Kinoshita, T. (1993) The cloning of PIG-A, a component in the early step of GPI-anchor biosynthesis. Science 259, 1318–1320.
Sims, J. E., March, C. J., Cosman, D., Widmer, M. B., MacDonald, H. R., McMahan, C. J., Grubin, C. E., Wignall, J. M., Jackson, J. L., Call, S. M., Friend, D., Alpert, A. R., Gillis, S., Urdal, D. L., and Dower, S. K. (1988) cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin superfamily. Science 241, 567–569.
Gearing, D., King, J., Gough, N., and Nicola, N., (1989) Expression cloning of a receptor for human granulocyte-macrophage colony-stimulating factor. EMBO J. 8, 3667–3676.
Elliott, J. F., Albrecht, G. R., Gilladoga, A., Handunnetti, S. M., Neequaye, J., Lallinger, G., Minjas, J. N., and Howard, R. J. (1990) Genes for Plasmodium falciparum Proc. Natl. Acad. Sci. USA 87, 6363–6367.
Gorman, C. (1986) High efficiency gene transfer into mammalian cells, in DNA Cloning (Grover, D. M., ed.), IRL, Oxford pp. 143–190.
Simmons, D. and Needham, L. A., (1992) Cloning cell-surface molecules using monoclonal antibodies, in Vascular Endothelium: Interactions with Circulating Cells (Gordon, J., ed.), Elsevier, Amsterdam pp. 3–29.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Humana Press Inc.
About this protocol
Cite this protocol
Simmons, D.L. (1999). Functional Analysis of Cell Adhesion Molecules. In: Dejana, E., Corada, M. (eds) Adhesion Protein Protocols. Methods in Molecular Biology, vol 96. Humana Press. https://doi.org/10.1385/1-59259-258-9:39
Download citation
DOI: https://doi.org/10.1385/1-59259-258-9:39
Publisher Name: Humana Press
Print ISBN: 978-0-89603-417-4
Online ISBN: 978-1-59259-258-6
eBook Packages: Springer Protocols