Abstract
p125FAK tyrosine phosphorylation is a primary response to integrin stimulation. The first evidence that integrin occupancy by ligands induces tyrosine phosphorylation came from studies on the fibrinogen receptor αllbβ3 in platelets (1). Further work showed that both in normal and transformed cells several different integrin receptors can trigger tyrosine phosphorylation of intracellular proteins following interactions with matrix proteins or integrin receptor clustering by specific antibodies (2).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ferrel. J. E. and Martin, G. S. (1989) Tyrosine-specific protein phosphorylation is regulated by glycoprotein llb-llla in platelets. Proc. Natl. Acad. Sci. USA 86, 2234–2238.
Clark, E. A. and Brugge, J. S. (1995) Integrins and signal transduction pathways: the road taken. Science 8, 233–239.
Kornberg, L., Earp, H. S., Parsons, J. T., Schaller, M., and Juliano R. L. (1992) Cell adhesion of integrin clustering increases phosphorylation of a focal adhesion associated tyrosine kinase. J. Biol. Chem. 267, 23,439–23,442.
Burridge, C. A., Turner, C., and Romer, L. (1992) Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. J. Cell. Biol. 119, 893–904.
Clark, E. A., Shattil, S. J., Ginsberg, M. H., Bolen, J., and Brugge, J. S. (1994) Regulation of the protein tyrosine kinase pp72syk by platelet agonists and the integrin alphallb/beta3. J. Biol. Chem. 269, 28,859–28,864.
Sakai, R., Iwamatsu, A., Hirano, N., Ogawa, S., Tanaka, T., Mano, H., Yazaki, Y., and Hirai, H. (1994) A novel signaling molecule, p130, forms stable complexes in vivo with v-crk and c-src in a tyrosine phosphorylation-dependent manner. EMBO J. 13, 3748–3756.
Nojima, Y., Tachibana, K., Sato, T., Schlossman, S. F., and Morimoto, C. (1995) Focal adhesion kinase is tyrosine phosphorylated after engagement of α4/β1 and α5/β1 integrins on human T-lymphoblastic cells. Cell Immunol. 161, 8–13.
Vuori, K. and Ruoslahti, E. (1995) Tyrosine phosphorylation of p130cas and cortactin accompanies integrin-mediated cell adhesion to extracellular matrix. J. Biol. Chem. 270, 22,259–22,262.
Hanks, S. K., Calalb, M. B., Harper, M. C., and Patel, S. K. (1992) Focal adhesion proteintyrosine kinase phosphorylated in response to cell attachment to fibronectin. Proc. Natl. Acad. Sci. USA 89, 8487–8491.
Schaller M. D., Borgman, C. A., Cobb. B. S., Vines, R. R., Reynolds, A. B. Parsons. J. T. (1992) pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions. Proc. Natl. Acad. Sci. USA 89, 5192–5196.
Defilippi, P., van Hinsberg, V., Bertolotto, A., Rossino, P., Silengo, L., and Tarone, G. (1991) Differential distribution and modulation of expression of alpha1/beta1 integrin on human endothelial cells. J. Cell. Biol. 114, 855–863.
Ey, P. L., Prowse, S. J., and Jenkin, C. R. (1978) Isolation of pure IgG1 and IgG2b immunoglobulins from mouse serum using Protein A-Sepharose. Immunochem. 15, 429–436.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Humana Press Inc.
About this protocol
Cite this protocol
Venturino, M., Olivo, C., Tarone, G., Defilippi, P. (1999). Induction of Intracellular Signals Through Binding of Adhesive Molecules. In: Dejana, E., Corada, M. (eds) Adhesion Protein Protocols. Methods in Molecular Biology, vol 96. Humana Press. https://doi.org/10.1385/1-59259-258-9:191
Download citation
DOI: https://doi.org/10.1385/1-59259-258-9:191
Publisher Name: Humana Press
Print ISBN: 978-0-89603-417-4
Online ISBN: 978-1-59259-258-6
eBook Packages: Springer Protocols