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Fluorescent Measurement of [Ca2+]c

Basic Practical Considerations

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Calcium Signaling Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 114))

Abstract

It is extremely difficult to write a prescriptive account of how to measure cytosolic-free Ca2+ ([Ca2+]c) that will suit all potential investigators, given the wide diversity of fluorescent Ca2+ indicators that are now available, the variety of cells to be investigated, and an increasing range of detection equipment that can be used. Therefore, this chapter is designed to provide the user with sufficient background in the technology so that he or she can move toward developing a protocol that will suit the cells, the experimental objectives, and the equipment available to the investigator.

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Authors and Affiliations

  1. Department of Human Anatomy and Cell Biology, University of Liverpool, Liverpool, UK

    Alec W. M. Simpson

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  1. Alec W. M. Simpson
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Editors and Affiliations

  1. University of Leicester, UK

    David G. Lambert

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© 1999 Humana Press Inc., Totowa, NJ

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Simpson, A.W.M. (1999). Fluorescent Measurement of [Ca2+]c . In: Lambert, D.G. (eds) Calcium Signaling Protocols. Methods in Molecular Biology™, vol 114. Humana Press. https://doi.org/10.1385/1-59259-250-3:3

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  • DOI: https://doi.org/10.1385/1-59259-250-3:3

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-597-3

  • Online ISBN: 978-1-59259-250-0

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