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Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations

  • Protocol
Calcium Signaling Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 114))

Abstract

Manipulation of cell function through stable transfection of agonist-specific receptors, components of second messenger cascades, or proteins with specific functional activities (enzymes, transporters, channels) has provided the tools to study intra- and intercellular signaling phenomena at a molecular level (1–4). In addition, cotransfection of cells with sequences coding for fluorescent markers such as green fluorescent protein has facilitated the selection and analysis of transfected cells (5). Techniques are also required to screen for and to monitor the functional changes associated with the genetic manipulation in order to investigate the physiological role of a component within a complex system such as a cell. Hormones and other agonists often elicit changes in intracellular pH (pHin) (6,7) and/or calcium ([Ca2+]i) (8,9) that modulate cell responses. Thus, methods for comparing steady-state ion concentrations and the regulation of these ions between cell populations in which signaling pathways have been modified can provide an approach to screen for functional changes in signal transduction for selective agonists.

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Author information

Authors and Affiliations

  1. Department of Physiology, Texas Tech University, Health Sciences Center, Lubbock, TX

    Raul Martinez-Zaguilan

  2. Department of Physiology, University of Arizona, Tucson, AZ

    Linda S. Tompkins

  3. Department of Biochemistry, University of Arizona, Arizona Health Sciences Center, Tucson, AZ

    Robert J. Gillies

  4. Department of Physiology, University of Arizona, Arizona Health Sciences Center, Tucson, AZ

    Robert J. Gillies & Ronald M. Lynch

Authors
  1. Raul Martinez-Zaguilan
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  2. Linda S. Tompkins
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  3. Robert J. Gillies
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  4. Ronald M. Lynch
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Editor information

Editors and Affiliations

  1. University of Leicester, UK

    David G. Lambert

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© 1999 Humana Press Inc., Totowa, NJ

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Martinez-Zaguilan, R., Tompkins, L.S., Gillies, R.J., Lynch, R.M. (1999). Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations. In: Lambert, D.G. (eds) Calcium Signaling Protocols. Methods in Molecular Biology™, vol 114. Humana Press. https://doi.org/10.1385/1-59259-250-3:287

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  • DOI: https://doi.org/10.1385/1-59259-250-3:287

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-597-3

  • Online ISBN: 978-1-59259-250-0

  • eBook Packages: Springer Protocols

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