Measurement of Intracellular Calcium Concentration Using Confocal Microscopy

  • Carmen Perez-Terzic
  • Marisa Jaconi
  • Lisa Stehno-Bittel
Part of the Methods in Molecular Biology™ book series (MIMB, volume 114)


Many cellular functions are tightly regulated by intracellular calcium concentrations ([Ca2+]i), and, therefore, the measurement of [Ca2+]i is of critical importance. To determine Ca2+-related cellular dynamics accurately, it is necessary to measure three-dimensionally resolved [Ca2+]i with sufficient temporal resolution to follow fast cellular responses that generate signal pulses and wave propagation. Several techniques have been developed to assay [Ca2+]i, but a revolution in the study of intracellular [Ca2+]i occurred when fluorescent dyes for Ca2+ were developed (1). Since then, fluorescent dyes and fluorescent microscopy have been used to observe resting and nonresting [Ca2+] in intact cells as well as in subcellular fractions.


Nuclear Envelope High Numerical Aperture Female Frog Sufficient Temporal Resolution Back Aperture 
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Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Carmen Perez-Terzic
    • 1
  • Marisa Jaconi
    • 2
  • Lisa Stehno-Bittel
    • 3
  1. 1.Departments of Medicine and Pharmacology, Division of Cardiovascular DiseasesMayo Clinic, Mayo FoundationRochester
  2. 2.Laboratoire de Physiopathologie CardiovasculaireINSERMMontpellierFrance
  3. 3.University of Kansas Medical CenterKansas City

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