Abstract
Integrins are heterodimeric cell-adhesion receptors that comprise a family of over 20 different combinations of αand βsubunits that function in diverse cellular processes, including development, growth, motility, metastasis, apoptosis, inflammation, hemostasis, and bone resorption (for review, see refs. 1,2). αvβ3 is a member of a subclass of integrins that show binding specificities to peptide sequences encoding an RGD (-Arg-Gly-Asp-) structural motif. Recent interest has focused on αvβ3, which has been shown to have important roles in tumor angiogenesis and metastasis (3,4), hyperplasia leading to restenosis (5), osteoclast-mediated bone resorption (6,7), and retinal neovascularization (8). Both in vitro and in vivo studies, employing specific antibodies to αvβ3, as well as short RGD-containing peptide sequences, have been used to demonstrate therapeutic utility in blocking the function of this receptor in animal models.
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© 1999 Humana Press Inc.
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Wu, J.M., Rosser, M.P., Howlett, A.R., Feldman, R.I. (1999). A Cell-Based Adhesion Assay for the Characterization of Integrin αvβ3 Antagonists. In: Howlett, A. (eds) Integrin Protocols. Methods in Molecular Biology, vol 129. Humana Press. https://doi.org/10.1385/1-59259-249-X:211
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DOI: https://doi.org/10.1385/1-59259-249-X:211
Publisher Name: Humana Press
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