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Detection of Mutations and Polymorphisms in Clotting Factors by Denaturing Gradient Gel Electrophoresis

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Hemostasis and Thrombosis Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 31))

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Abstract

In the last few years a number of methods have been developed that, in combination with the PCR method (1), have vastly improved the detection of mutations and polymorphisms. The most frequently used screening methods for mutation hunting in bleeding disorders are denaturing gradient gel electrophoresis (DGGE) (2), single-strand conformation polymorphism (SSCP) (3), and chemical mismatch cleavage (4). This chapter gives an introduction to the theory and practice of DGGE that facilitates the establishment of this mutation screening method.

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Authors and Affiliations

  1. Institut für Experimentelle HÄmatologie und Transfusions, Medizin der UniversitÄt Bonn, Bonn, Germany

    Rainer Schwaab & Winfried Schmidt

Authors
  1. Rainer Schwaab
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  2. Winfried Schmidt
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Editors and Affiliations

  1. Royal Free Hospital, London, UK

    David J. Perry  & K. J. Pasi  & 

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© 1999 Humana Press Inc.

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Schwaab, R., Schmidt, W. (1999). Detection of Mutations and Polymorphisms in Clotting Factors by Denaturing Gradient Gel Electrophoresis. In: Perry, D.J., Pasi, K.J. (eds) Hemostasis and Thrombosis Protocols. Methods in Molecular Medicine™, vol 31. Humana Press. https://doi.org/10.1385/1-59259-248-1:85

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  • DOI: https://doi.org/10.1385/1-59259-248-1:85

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-419-8

  • Online ISBN: 978-1-59259-248-7

  • eBook Packages: Springer Protocols

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