Abstract
Prothrombotic evaluation of patients with a history—and in particular a family history—of venous thromboembolic disease is becoming increasingly important as our understanding of the molecular abnormalities that underlie this clinical disorder increases. A recently described G→A polymorphism at position 20210 in the 3′-untranslated region of the prothrombin gene (F2 3′-UTR) has been found to be associated with an increased risk of venous thrombotic disease. In the Leiden Thrombophilia Study (LETS), the prevalence of carriers of the 20210 A allele in the healthy population was 2.3%, among patients with a single objectively proven DVT 6.2% and in a selected group of patients with a personal and family history of venous thrombosis 18%.
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References
Poort, S. W., Rosendaal, F. R., Retisma, P. H., and Bertina, R. M. (1996) A common genetic variation in the 3′-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis. Blood 88, 3698–3703.
Gandrille, S., Alhenc-Gelas, M., and Aiach, M. (1995) A rapid screening method for the Factor V Leiden Arg 506→Gln mutation. Blood Coag. Fibrinol. 6, 245–248.
Brown, K., Luddington, R., Williamson, D., Baker, P., and Baglin, T. (1997) Risk of venous thromboembolism associated with a G to A transition at position 20210 in the 3′-untranslated region of the prothrombin gene. Br. J. Haematol. 98, 907–909.
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© 1999 Humana Press Inc.
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Mellars, G., Jenkins, P.V., Perry, D.J. (1999). Multiplex PCR for Detection of the Prothrombin 3′-UTR (G20210A) Polymorphism and the Factor V Leiden Mutation. In: Perry, D.J., Pasi, K.J. (eds) Hemostasis and Thrombosis Protocols. Methods in Molecular Medicine™, vol 31. Humana Press. https://doi.org/10.1385/1-59259-248-1:287
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DOI: https://doi.org/10.1385/1-59259-248-1:287
Publisher Name: Humana Press
Print ISBN: 978-0-89603-419-8
Online ISBN: 978-1-59259-248-7
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