Direct Sequencing of PCR Products

  • David J. Perry
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 31)

Abstract

Sequencing is an increasingly important part of the analysis of a polymerase chain reaction (PCR) product and is used in many areas of hemostasis. The protocols described in this chapter have been used to sequence many different PCR templates and provide high-quality, reproducible sequence data.

Keywords

Vortex Sucrose Phenol Urea EDTA 

Suggested Reading

  1. Griffin, H. G. and Griffin, A. M. (1993) DNA Sequencing Protocols. Humana Press, Totowa, New Jersey.CrossRefGoogle Scholar
  2. McPherson, M. J., Hames, B. D., and Taylor, G. R. (1994) PCR 2: A Practical Approach. IRL, Oxford, UK.Google Scholar

Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • David J. Perry
    • 1
  1. 1.Department of Haematology, Haemophilia Centre and Haemostasis UnitRoyal Free Hospital School of MedicineLondonUK

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