Use of cDNA Representational Difference Analysis to Identify Disease-Specific Genes in Human Atherosclerotic Plaques
Changes in gene expression underlie many biological phenomena, including cellular differentiation and activation, embryonic development, and pathological processes. In recent years, much attention has been focused on the identification of genes that are differentially expressed between normal and disease states: the isolation of disease-specific genes is not only essential for our understanding of the molecular basis of pathological conditions, but may potentially highlight novel therapeutic targets.
KeywordsVortex Agarose MgCl2 Diethyl Isopropanol
- 2.Tyson, K. L., Weissberg, P. L., and Shanahan, C. M. Unpublished observations.Google Scholar
- 12.Patel, I. R., Attur, M. G., Patel, R. N., Stuchin, S. A., Abagyan, R. A., Abramson, S. B., and Amin, A. R. (1998) TNFα convertase enzyme from human arthritisaffected cartilage: Isolation of cDNA by differential display, expression of the active enzyme and regulation of TNFα. J. Immunol., 160, 4570–4579.PubMedGoogle Scholar
- 15.Gonzalez-Zulueta, M., Ensz, L. M., Mukhina, G., Lebovitz, R. M., Zwacka, R. M., Engelhardt, J.F., Oberley, L. W., Dawson., V. L., and Dawson, T. M. (1998) Manganese superoxide dismutase protects nNOS neurons from NMDA and nitric oxide-mediated neurotoxicity. J. Neurosci., 18, 2040–2055.PubMedGoogle Scholar
- 20.Gress, T. M., Wallrapp, C., Frohme, M., MullerPillasch, F., Lacher, U., Friess, H., Buchler, M., Adler, G., and Hoheisel, J. D. (1997) Identification of genes with specific expression in pancreatic cancer by cDNA representational difference analysis. Genes Chromosomes and Cancer 19, 97–103.CrossRefPubMedGoogle Scholar
- 22.Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd ed., Cold Spring Harbor Laboratory Press, New York.Google Scholar