Assays for Measuring Hepadnaviral Supercoiled DNA

  • Stephen A. Locamini
  • Gilda M. Civitico
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 24)


Amajor reason for treatment failure during antiviral therapy of chronic hepatitis B infection is thought to be the persistence of the key replicative intermediate, the viral covalently closed circular (CCC) or supercoiled DNA (1,2). Investigators studying the structure and function of hepadnaviral CCC DNA (3) have provided evidence that suggests that this structure exists in the nucleus of infected hepatocytes as a heterogeneous population of viral minichromosomes, which range from half to fully chromatinized, thought to be owing to their association with variable numbers of nucleosomes.


Southern Blot Hybridization Microfuge Tube Replicative Intermediate Infected Hepatocyte Southern Blot Hybridization Analysis 
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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Stephen A. Locamini
    • 1
  • Gilda M. Civitico
    • 2
  1. 1.Research and Molecular DevelopmentVictorian Infectious Diseases Reference LaboratoryNorth MelbourneAustralia
  2. 2.Victorian Infectious Diseases Reference LaboratoryFairfield HospitalFairfieldUK

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