Abstract
Approximately 5% of the world’s population has been infected with hepatitis B virus (HBV). Ten percent of these adults will become chronic carriers, as will 95% of the infants infected perinatally. Those that do become chronically infected with HBV are at increased risk of developing liver dysfunction, cirrhosis, and liver failure (1). In addition, they also have a greater incidence of heptocellular carcinoma (2). Approximately two million chronic carriers die annually from liver disease attributed to infection by HBV (3).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Dudley, F. J., Sheuer, P. J., and Shelock, S. (1972) Natural history of hepatitis associated antigen positive chronic liver disease. Lancet ii, 1388–1393.
Beasley, R. P. and Hwang, L.-Y. (1994) Epidemiology of heptocellular carcinoma, in Viral Hepatitis and Liver Disease (Vyas, G. N., Dienstag, J. L., and Hoofnagle, J. H., eds.), Grune & Stratton, New York, pp. 209–224.
World Health Organization (1990) WHO reports decry of neglect of world health problems. ASM News 56, 358,359.
Seeger, C., Summers, J., and Mason, W. S. (1991) Viral DNA synthesis, in Current Topics in Microbiology and Immunology, vol. 168. Springer-Verlag, Berlin,pp. 41–66.
Wang, G. H. and Seeger, C. (1993) Novel mechanism for reverse transcription in hepatitis B viruses. J. Virol. 67, 6507–6512.
Hirsch, R. C., Lavine, J. E., Chang, L.-J., Varmus, H. E., and Ganem, D. (1990) Polymerase gene products of hepatitis B viruses are required for genomic RNA packaging as well as for reverse transcription. Nature (London) 344, 552–555.
Wang, G. H. and Seeger, C. (1992) The reverse transcriptase of hepatitis B virus acts as a protein primer for viral DNA synthesis. Cell 71, 633–670.
Rossner, M. T. (1992) Hepatitis B virus X-gene product: a promising transcriptional activator. J. Med. Virol. 36, 101–117.
Ladner, S. K., Otto, M. J., Barker, C. S., Zaifert, K., Wang, G.-H., Guo, J.-T., Seeger, C., and King, R. W. (1997) Inducible expression of human hepatitis B virus in stably transfected hepatoblastoma cell: a novel system for screening potential inhibitors of HBV replication, submitted for publication.
Current Protocols in Molecular Biology, vol. 3 (1995) (Janssen, K., series ed.), Wiley, New York, pp. A.2.5.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc.
About this protocol
Cite this protocol
King, R.W., Ladner, S.K. (2000). Hep AD38 Assay. In: Kinchington, D., Schinazi, R.F. (eds) Antiviral Methods and Protocols. Methods in Molecular Medicine™, vol 24. Humana Press. https://doi.org/10.1385/1-59259-245-7:43
Download citation
DOI: https://doi.org/10.1385/1-59259-245-7:43
Publisher Name: Humana Press
Print ISBN: 978-0-89603-561-4
Online ISBN: 978-1-59259-245-6
eBook Packages: Springer Protocols