Quantitative Immunodetection of Viral Oncoprotein Levels in Human Papillomavirus-Transformed Human Cells

  • Jeffrey A. Johnson
  • J. David Gangemi
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 24)


Human papillomaviruses (HPV) comprise a genus in the species Papovaviridae, which consist of small, naked icosahedral viruses containing circular dsDNA. HPV types 16 and 18 infect squamous epithelium of the genitalia and may infect as many as 45% of the female population in developed countries (1).


ELISA Plate Reader Tumor Suppressor Gene Product Resuspend Cell Pellet Circular dsDNA Endogenous Alkaline Phosphatase Activity 
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    Peto, R. (1986) Introduction: geographic patterns and trends, in Viral Etiology of Cervical Cancer, Banbury report 21 (Peto, R. and ZurHausen, H., eds.), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 3–15.Google Scholar
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    Vousden, K. H. (1994) Interactions between papillomavirus proteins and tumor suppressor gene products. Adv. Cancer Res. 64, 1–24.CrossRefPubMedGoogle Scholar
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    Antinore, M. J., Birrer, M. J., Patel, D., Nader, L., and McCance, D. J. (1996) The human papillomavirus type 16 E7 gene product interacts with and trans-activates the AP1 family of transcription factors. EMBO J. 15, 1950–1960.PubMedGoogle Scholar
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    Johnson, J. A. and Gangemi, J. D. (1997) Data presented at the 10th International Conference on Antiviral Research, Atlanta, GA.Google Scholar

Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Jeffrey A. Johnson
  • J. David Gangemi

There are no affiliations available

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