Determination of Viral Infectivity

  • David R. Harper
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 24)


Compared to the wide range of antibiotics that are available, the number of antiviral drugs is limited. However, herpesviruses have always been a major target for antiviral drug design, and there are a wide range of drugs at various stages of development. All of the assays described in this chapter provide relatively simple methods for plaque reduction assay of herpesviruses. Clearly, it is not possible to perform such assays on those herpesviruses that do not grow in adherent cells, such as Epstein-Barr virus. These require a different approach and are discussed elsewhere in this volume.


Methylene Blue Maintenance Medium Sodium Hydrogen Carbonate Plaque Reduction Assay Overlay Medium 
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  1. 1.
    Welsh, M. D. (1991) Studies on the neutralisation of VZV by two specific monoclonal antibodies. Honours thesis, Queens University of Belfast, Belfast, UK.Google Scholar
  2. 2.
    Grose, C. and Brunel, P. A. (1978) Varicella-zoster virus: isolation and propagation in human melanoma cells at 36 and 32 degrees C. Infect. Immun. 19, 199–203.PubMedGoogle Scholar
  3. 3.
    Harper, D. R., Gilbert, R. L., O’Connor, T. J., Kinchington, D., Mahmood, N., McIlhinney, R. A. J., and Jeffries, D. J. (1996) Antiviral activity of 2-hydroxy fatty acids. Antivir. Chem. Chemother. 7, 138–141.Google Scholar

Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • David R. Harper
    • 1
  1. 1.Department of VirologySt. Bartholomew’s and the Royal London School of Medicine and DentistryLondonUK

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