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Analysis and Reconstitution of Chlorophyll-Proteins

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Heme, Chlorophyll, and Bilins

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Abstract

It is hard to believe that only some 30 years ago, it was a matter of debate whether chlorophyll (Chl) and other photosynthesis pigments are protein-bound or just dissolved in plant membranes. Philip Thornber, who vividly described this debate in his recollection of photosynthesis research in the 1960s (90), was one of the exponents who finally convinced their colleagues that most, if not all, Chl in plants is in fact organized in protein complexes. It was his laboratory that devised quite a number of chromatographic and electrophoretic techniques for isolating (bacterio)chlorophyll-containing complexes from bacteria and plants. These isolation techniques later paved the way for structural analyses of, e.g., photosynthetic reaction centers of purple bacteria (24) as well as bacterial (53) and plant (49) light-harvesting complexes (LHC).

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  1. Institut für Allgemeine Botanik der Johannes-Gutenberg, Universität Mainz, Mainz, Germany

    Harald Paulsen & Volkmar H. R. Schmid

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  1. Harald Paulsen
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  1. Department of Plant Sciences, University of Cambridge, Cambridge, UK

    Alison G. Smith

  2. Department of Biochemistry, University of Cambridge, Cambridge, UK

    Michael Witty

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Paulsen, H., Schmid, V.H.R. (2002). Analysis and Reconstitution of Chlorophyll-Proteins. In: Smith, A.G., Witty, M. (eds) Heme, Chlorophyll, and Bilins. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-243-0:235

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  • DOI: https://doi.org/10.1385/1-59259-243-0:235

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-111-0

  • Online ISBN: 978-1-59259-243-2

  • eBook Packages: Springer Book Archive

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