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Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development

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Embryonic Stem Cells

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 185))

Abstract

With the attractive potential therapeutic uses of both endothelial and hematopoietic cells in medicine, much attention has been focused upon the differentiation of embryonic stem (ES) cells into mature cells of these lineages. In this chapter, we present a culture system that has successfully generated both complex endothelial structures and mature hematopoietic cells from undifferentiated ES cells in vitro. The simplicity of this system allows detailed analyses of factors and developmental steps essential in the generation of vascular structures and mature hematopoietic cell types.

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Authors and Affiliations

  1. Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto, Japan

    Stuart T. Fraser, Minetaro Ogawa, Satomi Nishikawa & Shin-Ichi Nishikawa

Authors
  1. Stuart T. Fraser
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  2. Minetaro Ogawa
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  3. Satomi Nishikawa
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  4. Shin-Ichi Nishikawa
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Editor information

Editors and Affiliations

  1. Ottawa Health Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2002 Humana Press Inc., Totowa, NJ

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Fraser, S.T., Ogawa, M., Nishikawa, S., Nishikawa, SI. (2002). Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development. In: Turksen, K. (eds) Embryonic Stem Cells. Methods in Molecular Biology™, vol 185. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-241-4:71

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  • DOI: https://doi.org/10.1385/1-59259-241-4:71

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-881-3

  • Online ISBN: 978-1-59259-241-8

  • eBook Packages: Springer Protocols

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