Murine Embryonic Stem Cells as a Model for Stress Proteins and Apoptosis During Differentiation
The early phase of murine embryonic stem (ES) cell differentiation is accompanied with the expression of proteins that are key players in this phenomenon. The function of some these proteins can be analyzed by using the classical “knock-out gene technology”, that consists of the inactivation of the endogenous gene that encodes the studied protein. This approach can be considered if the analyzed protein does not play a vital role; in other words, if the cells can stillundergo differentiation without expressing the protein in question. To analyze proteins whose expression is essential during early differentiation, an antisense strategy can be used that interferes with the expression of the studied protein. One interesting protein we studied with this technique is the small stress protein Hsp27 (1).
KeywordsEmbryonic Stem Cell Leukemia Inhibitor Factor Hsp27 Expression Embryonic Stem Cell Differentiation Murine Embryonic Stem Cell
- 1.Arrigo, A.-P. (1998) Small stress proteins: chaperones that act as regulators of intracellular redox and programmed cell death. Biol. Chem. Hoppe Seyler 379, 19–26.Google Scholar
- 4.Chaufour, S., Mehlen, P., and Arrigo, A.-P. (1996) Transient accumulation, phosphorylation and changes in the oligomerization of Hsp27 during retinoic acid-induced differentiation of HL-60 cells: possible role in the control of cellular growth and differentiation. Cell Stress Chaperones 4, 225–235.CrossRefGoogle Scholar
- 6.Mehlen, P., Preville, X., Chareyron, P., Briolay, J., Klemenz, R., and Arrigo, A.-P. (1995) Constitutive expression of human hsp27, Drosophila hsp27 and human α-Bcrystallin confers resistance to tumor necrosis factor-and oxidative stress-induced cytotoxicity in stably transfected murine L929 fibroblasts. J. Immunol. 154, 363–374.PubMedGoogle Scholar
- 8.Wang, R., Clark, R., and Bautch, V. L. (1992) Embryonic stem cell-derived cystic embryonic bodies from vascular channels: an in vitro model of blood vessel development. Dev. 114, 303–316.Google Scholar