Murine Embryonic Stem Cells as a Model for Stress Proteins and Apoptosis During Differentiation

  • André-Patrick Arrigo
  • Patrick Mehlen
Part of the Methods in Molecular Biology™ book series (MIMB, volume 185)

Abstract

The early phase of murine embryonic stem (ES) cell differentiation is accompanied with the expression of proteins that are key players in this phenomenon. The function of some these proteins can be analyzed by using the classical “knock-out gene technology”, that consists of the inactivation of the endogenous gene that encodes the studied protein. This approach can be considered if the analyzed protein does not play a vital role; in other words, if the cells can stillundergo differentiation without expressing the protein in question. To analyze proteins whose expression is essential during early differentiation, an antisense strategy can be used that interferes with the expression of the studied protein. One interesting protein we studied with this technique is the small stress protein Hsp27 (1).

Keywords

Codon Agarose Electrophoresis MgCl2 Polypeptide 

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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • André-Patrick Arrigo
    • 1
  • Patrick Mehlen
    • 2
  1. 1.Laboratoire du Stress Oxydant, Chaperons et Apoptose, Center de Genetique Moleculaire et CellulaireUniversity Claude Bernard Lyon-IVilleurbanneFrance
  2. 2.Laboratoire Différenciation et Apoptose, CNRSUniversité Claude Bernard Lyon-IFrance

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