Abstract
A two-step strategy for changing the specificity of antibodies (Abs) is presented, which we have used to change the specificity of an Ab from 11-deoxycortisol (11-DOC) to cortisol (CS). Two kinds of in vitro mutagenesis are utilized in this protocol: first, mutations are introduced at restricted positions in the complementarity-determining regions (CDRs) by site-directed mutagenesis; second, mutations are introduced into the entire V-coding regions by random mutagenesis.
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References
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© 2002 Humana Press Inc.
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Iba, Y., Miyazaki, C., Kurosawa, Y. (2002). Two-Step Strategy for Alteration of Immunoglobulin Specificity by In Vitro Mutagenesis. In: O’Brien, P.M., Aitken, R. (eds) Antibody Phage Display. Methods in Molecular Biology™, vol 178. Humana Press. https://doi.org/10.1385/1-59259-240-6:259
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DOI: https://doi.org/10.1385/1-59259-240-6:259
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