Abstract
Cell surfaces provide a rich source of potential antigen (Ag) targets for therapeutic and research reagent antibodies (Abs). However, in some circumstances, access to these targets may be difficult since it is technically challenging to purify individual Ags while retaining their native configuration. One way to circumvent the need for purification is to use whole cells or cell membranes as the basis for Ab selection. This has, in a number of cases, been successful, but necessitates the need for large-scale screening processes because the selection process will also generate many Abs that are not specific for the target of interest, but which bind to other proteins on the cell surface. Proximity (ProxiMol) selection is a method of selection that enriches the selected population for Abs that bind at or around sites on the cell surface of the target Ag and so reduce the need for labor-intensive screening processes.
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References
Bobrow, M. N., Harris, T. D., Shaughnessy, K. J., and Litt, G. J. (1989) Catalyzed reporter deposition, a novel method of signal amplification. J. Immunol. Methods 125, 279–285.
Bobrow, M. N., Litt, G. J., Shaughnessy, K. J., Mayer, P. C., and Colon, J. (1992) The use of catalyzed reporter deposition as a means of signal amplification in a variety of formats. J. Immunol. Methods 150, 145–149.
Merz, H., Malisius, R., Mannweiler, S., Zhou, R., Hartmann, W., Orscheschek, K., Moubayed, P., and Feller, A. C. (1995) A maximised immunohistochemical method for the retrieval and enhancement of hidden antigens. Lab. Invest. 73, 149–156.
Adams, J. C. (1992) Biotin amplification of biotin and horseradish. J. Histochem. Cytochem. 40, 1457–1463.
Earnshaw, J. C. and Osbourn, J. K. (1999) Signal amplification in flow cytometry using biotin tyramine. Cytometry 35, 176–179.
Osbourn, J. K., Derbyshire, E. J., Vaughan, T. J., Field, A. W., and Johnson, K. S. (1998) Pathfinder selection: in situ isolation of novel antibodies. Immunotechnology 3, 293–302.
Osbourn, J. K., Earnshaw, J. C., Johnson, K. S., Parmentier, M., Timmermans, V., and McCafferty, J. (1998) Directed selection of MIP-1α neutralizing CCR5 antibodies from a phage display human antibody library. Nature Biotechnol. 16, 778–781.
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© 2002 Humana Press Inc.
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Osbourn, J.K. (2002). Proximity-Guided (ProxiMol) Antibody Selection. In: O’Brien, P.M., Aitken, R. (eds) Antibody Phage Display. Methods in Molecular Biology™, vol 178. Humana Press. https://doi.org/10.1385/1-59259-240-6:201
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DOI: https://doi.org/10.1385/1-59259-240-6:201
Publisher Name: Humana Press
Print ISBN: 978-0-89603-906-3
Online ISBN: 978-1-59259-240-1
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