Abstract
In recent years, a number of single-pot antibody (Ab) libraries have been described, which permit the rapid isolation of high-affinity Abs against large panels of antigens (Ags). Naïve libraries have been generated by tapping the natural primary (unselected) immune repertoire via cloning of Abs that recognize a variety of Ags (1,2). The rearranged V genes were amplified with the polymerase chain reaction (PCR) from B-cell mRNAs encoding immunoglobulin M (IgM) taken from nonimmunized donors. By using this procedure, Abs were recovered prior to encounter with Ag and unscreened for tolerance by the immune system. Indeed, a naïve library represents a good source of Abs against self, nonimmunogenic, and toxic Ags if the library is sufficiently large and diverse.
Keywords
- Phage Particle
- Helper Phage
- Guanidine Isothiocyanate
- Moloney Murine Leukemia Virus Reverse Transcriptase
- Polyethylene Glycol Precipitation
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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© 2002 Humana Press Inc.
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de Haard, H.J. (2002). Construction of Large Naïve Fab Libraries. In: O’Brien, P.M., Aitken, R. (eds) Antibody Phage Display. Methods in Molecular Biology™, vol 178. Humana Press. https://doi.org/10.1385/1-59259-240-6:087
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DOI: https://doi.org/10.1385/1-59259-240-6:087
Publisher Name: Humana Press
Print ISBN: 978-0-89603-906-3
Online ISBN: 978-1-59259-240-1
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