Colony Hybridization of Bacterial Isolates with Burkholderia cepacia-Specific Probes
Colony hybridization is a very powerful tool for the examination of bacterial isolates that have been cultured. The advantages are that it can be used rather rapidly with a high degree of specificity to look at features of large numbers of isolates (1). For Burkholderia (formerly Pseudomonas) cepacia, species-specific probes that target the 16S and 23S rDNA are available and can be easily used in colony hybridization (2 and 3, respectively). Researchers are interested in B. cepacia for several reasons: it is abundant in nature (4, 5, 6), it is valuable in bioremediation and biocontrol (7), and it is clinically important, causing disease in cystic fibrosis patients and other compromised individuals (8).
KeywordsDust EDTA Cobalt Rubber Phenyl
- 3.Schleifer, K. H., Amann, R., Ludwig, E., Rothemund, C., Springer, N., and Dorn, S. (1992) Nucleic acid probes for the identification and in situ detection of Pseudomonas. In Pseudomonas: Molecular Biology and Biotechnology (Galli, E., Silver, S., and Withold, B., eds.), American Society for Microbiology, Washington, DCGoogle Scholar
- 10.MuKwaya, G. M. and Welch, D. F. (1989) Subgrouping of Pseudomonas cepacia by cellular fatty acid composition. J. Clin. Microbiol. 27, 2646–2649.Google Scholar