Gene Probes pp 23-25 | Cite as

Nick Translation

  • Alex Reid
Part of the Methods in Molecular Biology book series (MIMB, volume 179)


Nick translation was the first method devised for the in vitro labeling of DNA (1). During the reaction the DNA to be labeled is nicked by DNase I yielding a free 3′ hydroxyl end. DNA polymerase I then adds a new nucleotide to this end. The 5′–3′ exonuclease activity of the polymerase then moves the “nick” along the strand in the 3′ direction. The addition of a radioactively labeled nucleotide to the reaction results in probes that can be used in hybridization reactions to DNA immobilized on Southern blots, colony lifts, etc.


Radioactive Waste Glass Wool Nick Translation Exonuclease Activity Hybridization Reaction 
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  1. 1.
    Kelly, R. B., Cozzarelli, N. R., Deutscher, M. P., Lehman, I. R., and Kornberg, A. (1970) Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. J. Biol. Chem. 245, 39–43.PubMedGoogle Scholar

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© Humana Press Inc. 2002

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  • Alex Reid

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