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Genomics Protocols pp 323–340Cite as

Expression Monitoring Using cDNA Microarrays

A General Protocol

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 175))

Abstract

As the Human Genome Project nears the completion of the first human sequence, the next great challenge is to elucidate the function of these genes. One route of exploring the function of a gene is by determining its pattern of expression. Various methods are available for detecting and quantitating gene expression levels, including Northern blots (1), RNase protection assays (2), differential display (3), representational difference analysis (1), and serial analysis of gene expression (5). cDNA microarray technology (6,7) distinguishes itself from the other methods by allowing one to measure the expression levels of tens of thousands of genes in a single experiment. This capacity allows the expression of entire genomes to be monitored in parallel during different stages of embryonic development, disease progress, or drug response. Microarray technology has therefore attracted a great deal of interest from both academic and commercial sectors.

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Author information

Authors and Affiliations

  1. Moleular Biology Systems Analysis, LumiCyte, Inc., Fremont, CA

    Xing Jian Lou

  2. Department of Biochemistry, Beckman Center, Stanford University School of Medicine, Stanford, CA

    Mark Schena & Ronald W. Davis

  3. Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA

    Frank T. Horrigan

  4. Falk Cardiovascular Research Center, Stanford University School of Medicine, Stanford, CA

    Richard M. Lawn

Authors
  1. Xing Jian Lou
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  2. Mark Schena
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  3. Frank T. Horrigan
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  4. Richard M. Lawn
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  5. Ronald W. Davis
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Editor information

Editors and Affiliations

  1. UK Human Genome Mapping Project Resource Centre, Cambridge, UK

    Michael P. Starkey  & Ramnath Elaswarapu  & 

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© 2001 Humana Press Inc., Totowa, NJ

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Cite this protocol

Lou, X.J., Schena, M., Horrigan, F.T., Lawn, R.M., Davis, R.W. (2001). Expression Monitoring Using cDNA Microarrays. In: Starkey, M.P., Elaswarapu, R. (eds) Genomics Protocols. Methods in Molecular Biology™, vol 175. Humana Press. https://doi.org/10.1385/1-59259-235-X:323

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  • DOI: https://doi.org/10.1385/1-59259-235-X:323

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-774-8

  • Online ISBN: 978-1-59259-235-7

  • eBook Packages: Springer Protocols

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