Skip to main content

Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos

  • Protocol
Genomics Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 175))

Abstract

The genome of a given organism is considered in biology as the fundamental invariant (1). It is virtually the same throughout lifetime and, to a lesser extent, over generations. By contrast, genetic information is expressed in complex and ever-changing temporal and spatial patterns throughout development and differentiation. The description and analysis of these patterns is crucial to elucidate the functions of genes and to understand the network of genetic interactions that underlies the process of normal development.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Monod, J. (1970) Le hasard et la necessitè, Editions du Seuil, Paris.

    Google Scholar 

  2. Bettenhausen, B. and Gossler, A. (1995). Efficient isolation of novel mouse genes differentially expressed in early postimplantation embryos. Genomics 28, 436–441.

    Article  PubMed  CAS  Google Scholar 

  3. Gawantka, V., Pollet, N., Delius, H., Vingron, M., Pfister, R., Nitsch, R., Blumenstock, C., and Mehrs, C. (1998) Gene expression screening in Xenopus identifies molecular pathways, predicts gene function and provides a global view of embryonic patterning. Mech. Dev. 77, 95–141.

    Article  PubMed  CAS  Google Scholar 

  4. Kopczynski, C. C., Noordermeer, J. M., Serano, T. L., Chen, W.-C., Pendleton, J. D., Lewis, S., Goodman, C. S., and Rubin, G. M. (1998) A high throughput screen to identify secreted and transmembrane proteins involved in Drosophila embryogenesis. Proc. Natl. Acad. Sci. USA 95, 9973–9978.

    Article  PubMed  CAS  Google Scholar 

  5. Plickert, G., Gajewski, M., Gehrke, G., Gausepohl, H., Schlossherr, J., and Ibrahim, H. (1997) Automated in situ detection (AISD) of biomolecules. Dev. Gene. Evol. 207, 362–367.

    Article  CAS  Google Scholar 

  6. Niehrs, C. (1997) Gene expression screens in vertebrate embryos: more than meets the eye. Genes Funct. 1, 229–231.

    Article  PubMed  CAS  Google Scholar 

  7. Komiya, T., Tanigawa, Y., and Hirohashi, S. (1997) A large scale in situ hybridisation system using an equalised cDNA library. Anal. Biochem. 254, 23–30.

    Article  PubMed  CAS  Google Scholar 

  8. Wilkinson, D.G. and Nieto, M.A. (1993) Detection of messenger RNA by in situ hybridization to tissue sections and whole mounts. Methods Enzymol. 225, 361–373.

    Article  PubMed  CAS  Google Scholar 

  9. Harland, R. M. (1991) In situ hybridization: an improved whole-mount method for Xenopus embryos. Methods Cell Biol. 36, 685–695.

    Article  PubMed  CAS  Google Scholar 

  10. Wang, K., Gan, L., Boysen, C., and Hood L. (1995) A microtiter plate-based high-throughput DNA purification method. Anal. Biochem. 226, 85–90.

    Article  PubMed  CAS  Google Scholar 

  11. Islam, N. and Moss, T. (1996) Enzymatic removal of vitelline membrane and other protocol modifications for whole mount in situ hybridization of Xenopus embryos. Trends Genet. 12, 459.

    Article  PubMed  CAS  Google Scholar 

  12. Nieuwkoop, P. D. and Faber, J. (1984) Normal table of Xenopus laevis (Daudin), 2nd ed., Garland Publishing Inc., New York and London.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Pollet, N., Niehrs, C. (2001). Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos. In: Starkey, M.P., Elaswarapu, R. (eds) Genomics Protocols. Methods in Molecular Biology™, vol 175. Humana Press. https://doi.org/10.1385/1-59259-235-X:309

Download citation

  • DOI: https://doi.org/10.1385/1-59259-235-X:309

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-774-8

  • Online ISBN: 978-1-59259-235-7

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics