Abstract
The genome of a given organism is considered in biology as the fundamental invariant (1). It is virtually the same throughout lifetime and, to a lesser extent, over generations. By contrast, genetic information is expressed in complex and ever-changing temporal and spatial patterns throughout development and differentiation. The description and analysis of these patterns is crucial to elucidate the functions of genes and to understand the network of genetic interactions that underlies the process of normal development.
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References
Monod, J. (1970) Le hasard et la necessitè, Editions du Seuil, Paris.
Bettenhausen, B. and Gossler, A. (1995). Efficient isolation of novel mouse genes differentially expressed in early postimplantation embryos. Genomics 28, 436–441.
Gawantka, V., Pollet, N., Delius, H., Vingron, M., Pfister, R., Nitsch, R., Blumenstock, C., and Mehrs, C. (1998) Gene expression screening in Xenopus identifies molecular pathways, predicts gene function and provides a global view of embryonic patterning. Mech. Dev. 77, 95–141.
Kopczynski, C. C., Noordermeer, J. M., Serano, T. L., Chen, W.-C., Pendleton, J. D., Lewis, S., Goodman, C. S., and Rubin, G. M. (1998) A high throughput screen to identify secreted and transmembrane proteins involved in Drosophila embryogenesis. Proc. Natl. Acad. Sci. USA 95, 9973–9978.
Plickert, G., Gajewski, M., Gehrke, G., Gausepohl, H., Schlossherr, J., and Ibrahim, H. (1997) Automated in situ detection (AISD) of biomolecules. Dev. Gene. Evol. 207, 362–367.
Niehrs, C. (1997) Gene expression screens in vertebrate embryos: more than meets the eye. Genes Funct. 1, 229–231.
Komiya, T., Tanigawa, Y., and Hirohashi, S. (1997) A large scale in situ hybridisation system using an equalised cDNA library. Anal. Biochem. 254, 23–30.
Wilkinson, D.G. and Nieto, M.A. (1993) Detection of messenger RNA by in situ hybridization to tissue sections and whole mounts. Methods Enzymol. 225, 361–373.
Harland, R. M. (1991) In situ hybridization: an improved whole-mount method for Xenopus embryos. Methods Cell Biol. 36, 685–695.
Wang, K., Gan, L., Boysen, C., and Hood L. (1995) A microtiter plate-based high-throughput DNA purification method. Anal. Biochem. 226, 85–90.
Islam, N. and Moss, T. (1996) Enzymatic removal of vitelline membrane and other protocol modifications for whole mount in situ hybridization of Xenopus embryos. Trends Genet. 12, 459.
Nieuwkoop, P. D. and Faber, J. (1984) Normal table of Xenopus laevis (Daudin), 2nd ed., Garland Publishing Inc., New York and London.
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© 2001 Humana Press Inc., Totowa, NJ
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Pollet, N., Niehrs, C. (2001). Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos. In: Starkey, M.P., Elaswarapu, R. (eds) Genomics Protocols. Methods in Molecular Biology™, vol 175. Humana Press. https://doi.org/10.1385/1-59259-235-X:309
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DOI: https://doi.org/10.1385/1-59259-235-X:309
Publisher Name: Humana Press
Print ISBN: 978-0-89603-774-8
Online ISBN: 978-1-59259-235-7
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