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Genomics Protocols pp 279–294Cite as

Isolation of Differentially Expressed Genes by Representational Difference Analysis

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 175))

Abstract

Representational difference analysis of cDNA (cDNA RDA) is designed to compare two different mRNA populations resulting in the isolation of differentially expressed genes. RDA was originally developed for genomic DNA as a method to isolate the differences between two complex genomes (1) and later was adapted for cDNA to examine differential gene expression (2).

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References

  1. Lisitsyn, N. and Wigler, M. (1993) Cloning the differences between two complex genomes. Science 259, 946–951.

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  2. Hubank, M. and Schatz, D. G. (1994) Identifying differences in mRNA expression by representational difference analysis of cDNA. Nucleic Acids Res. 22, 5640–5648.

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  6. Sambrook, J., Fritsch, E. F., and Maniatis, T., eds. (1989) Molecular cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

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Author information

Authors and Affiliations

  1. Department of Internal Medicine I, University of Ulm, Ulm, Germany

    Christine Wallrapp & Thomas M. Gress

Authors
  1. Christine Wallrapp
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  2. Thomas M. Gress
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Editor information

Editors and Affiliations

  1. UK Human Genome Mapping Project Resource Centre, Cambridge, UK

    Michael P. Starkey  & Ramnath Elaswarapu  & 

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© 2001 Humana Press Inc., Totowa, NJ

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Cite this protocol

Wallrapp, C., Gress, T.M. (2001). Isolation of Differentially Expressed Genes by Representational Difference Analysis. In: Starkey, M.P., Elaswarapu, R. (eds) Genomics Protocols. Methods in Molecular Biology™, vol 175. Humana Press. https://doi.org/10.1385/1-59259-235-X:279

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  • DOI: https://doi.org/10.1385/1-59259-235-X:279

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-774-8

  • Online ISBN: 978-1-59259-235-7

  • eBook Packages: Springer Protocols

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