Low-Resolution Typing of HLA-DQA1 Using DNA Microarray
Over the last few decades, typing for the human leukocyte antigen (HLA) has become critical for bone marrow transplants. Research has also indicated that individuals with certain allelic genotypes may be at a higher risk for developing diseases affecting the immune system, and therefore rapid tests to identify the numerous allelic differences are needed. Such tests must allow for the detection of the various alleles present in a 4-Mbp region on the short arm of chromosome 6. The area comprises three class regions: class I, which includes HLA-A, HLA-B, and HLA-C genes; class II, containing the HLA-D genes; and class III, composed of numerous protein coding genes responsible for a range of functions (1,2).
KeywordsHuman Leukocyte Antigen Human Leukocyte Antigen Class Sodium Dodecyl Sulfate Solution Saline Sodium Citrate Human Leukocyte Antigen Typing
- 1.Trowsdale, J. (1996). Molecular genetics of HLA class 1 and Class II regions, in HLA and MHC Genes, Molecules, and Function (Browning, M. and McMichael, A., eds.), BIOS Scientific Publishers, Oxford, pp. 23–38.Google Scholar
- 2.Aguado, B., Milner, C. M., and Campbell, R. D. (1996) Genes of the MHC class III region and the function of the proteins they encode, in HLA and MHC Genes, Molecules, and Function. (Browning, M., and McMichael, A., eds.), BIOS Scientific Publishers, Oxford, pp. 39–75.Google Scholar
- 5.Robinson, J. (2000) IMGT/HLA Database Statistics <http://www3.ebi.ac.uk/Ser-vices/imgt/hla/cgi-bin/statistics.cgi>