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DNA Arrays pp 201-210 | Cite as

Low-Resolution Typing of HLA-DQA1 Using DNA Microarray

  • Sarah H. Haddock
  • Christine Quartararo
  • Patrick Cooley
  • Dat D. Dao
Part of the Methods in Molecular Biology™ book series (MIMB, volume 170)

Abstract

Over the last few decades, typing for the human leukocyte antigen (HLA) has become critical for bone marrow transplants. Research has also indicated that individuals with certain allelic genotypes may be at a higher risk for developing diseases affecting the immune system, and therefore rapid tests to identify the numerous allelic differences are needed. Such tests must allow for the detection of the various alleles present in a 4-Mbp region on the short arm of chromosome 6. The area comprises three class regions: class I, which includes HLA-A, HLA-B, and HLA-C genes; class II, containing the HLA-D genes; and class III, composed of numerous protein coding genes responsible for a range of functions (1,2).

Keywords

Human Leukocyte Antigen Human Leukocyte Antigen Class Sodium Dodecyl Sulfate Solution Saline Sodium Citrate Human Leukocyte Antigen Typing 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    Aguado, B., Milner, C. M., and Campbell, R. D. (1996) Genes of the MHC class III region and the function of the proteins they encode, in HLA and MHC Genes, Molecules, and Function. (Browning, M., and McMichael, A., eds.), BIOS Scientific Publishers, Oxford, pp. 39–75.Google Scholar
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    Begovich, A. B. and Erlich, H. A. (1995) HLA typing for bone marrow transplantation. J. Amer. Med. Assoc. 273, 586–591.CrossRefGoogle Scholar
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    Bodmer, J. G., Marsh, S. G. E., Albert, E. D., Bodmer, W. F., Bontrop, R. E., Charron, D., et al. (1995) Nomenclature for factors of the HLA system. Tissue Antigens 46, 1–18.PubMedCrossRefGoogle Scholar
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    Robinson, J. (2000) IMGT/HLA Database Statistics <http://www3.ebi.ac.uk/Ser-vices/imgt/hla/cgi-bin/statistics.cgi>

Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Sarah H. Haddock
    • 1
  • Christine Quartararo
    • 1
  • Patrick Cooley
    • 2
  • Dat D. Dao
    • 1
  1. 1.DNA Technology LaboratoryHouston Advanced Research CenterThe Woodlands
  2. 2.MicroFab Technologies Inc.Plano

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