Abstract
Pulmozyme® recombinant human deoxyribonuclease I (DNase I) is currently used as a therapeutic for cystic fibrosis (CF) (1) and may be effective in the treatment of systemic lupus erythematosus (SLE) (2,3). As described in Chapter 20, degradation of high-molecular-weight DNA following inhalation of DNase I as an aerosol decreases the viscoelasticity of CF sputum and improves lung function. In SLE, antibodies to DNA and DNA/anti-DNA immune complexes have been implicated as the principal causative factor underlying clinical pathogenesis (4–7). Degradation of extracellular DNA or DNA/anti-DNA immune complexes may be of clinical benefit in SLE by two mechanisms. First, hydrolysis of the DNA component of membrane deposited DNA/anti-DNA immune complexes may reduce inflammation in the affected tissues. Second, hydrolysis of DNA and/or DNA/anti-DNA immune complexes in the circulation may elicit a decrease in the production of antibodies to DNA over time by reducing the antigen load (8).
Keywords
- Systemic Lupus Erythematosus
- Cystic Fibrosis
- Ethidium Bromide Solution
- Cystic Fibrosis Sputum
- DNase Buffer
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Sinicropi, D.V., Lazarus, R.A. (2001). Assays for Human DNase I Activity in Biological Matrices. In: Schein, C.H. (eds) Nuclease Methods and Protocols. Methods in Molecular Biology™, vol 160. Humana Press. https://doi.org/10.1385/1-59259-233-3:325
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DOI: https://doi.org/10.1385/1-59259-233-3:325
Publisher Name: Humana Press
Print ISBN: 978-0-89603-679-6
Online ISBN: 978-1-59259-233-3
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