Abstract
Adipose tissue (AT) makes up 10–30% of body weight in normal-weight individuals, but its mass can vary from as little as 3–5% of body wt in lean, athletic subjects to 70% of body wt in very obese subjects.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Gurr, M. L. and Kirtland, J. (1978) Adipose tissue cellularity: a review. 1. Techniques for studying cellularity. Int. J. Obes. 2, 401–427.
DiGirolamo, M., Mendlinger, S., and Fertig, J. W. (1971) A simple method to determine fat cell size and number in four mammalian species. Am. J. Physiol. 221, 850–858.
Cushman, S. W. and Salans, L. B. (1978) Determination of adipose cell size and number in suspensions of isolated rat and human adipose cells. J. Lipid Res. 19, 269–273.
Smith, U., Sjöström, L., and Björntorp, P. (1972) Comparison of two methods for determining human adipose cell size. J. Lipid Res. 13, 822–824.
Dole, V. P. (1955) A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J. Clin. Invest. 35, 150–154.
Hirsch, J. and Gallian, E. (1968) Methods for the determination of adipose cell size in man and animals. J. Lipid Res. 9, 110–119.
Cartwright, A. L. (1987) Determination of adipose tissue cellularity, in Biology of the Adipocyte: Research Approaches. (Hausman, G. H. and Martin, R. J., eds.), Van Nostrand Reinhold, pp. 229–254.
Cooper, K. (1988) Neutralization of osmium tetroxide in case of spillage and for disposal. Bull. Microscop. Soc. Canada 8, 24–28.
Goldrick, R. B. (1967) Morphological changes in the adipocyte during fat deposition and mobilization. Am. J. Physiol. 212, 777–782.
Folch, J., Lees, M., and Stanley, G. H. S. (1957) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497–509.
Maroni, B. J., Haesemeyer, R., Wilson, L. K., and DiGirolamo, M. (1990) Electronic determination of size and number in isolated unfixed adipocyte populations. J. Lipid Res. 31, 1703–1709.
DiGirolamo, M. and Mendlinger, S. (1971) Role of fat cell size and number in enlargement of epididymal fat pads in three species. Am. J. Physiol. 221, 859–864.
DiGirolamo, M. (1972) Fat cell size, metabolic capacities and hormonal responsiveness of adipose tissue in spontaneous obesity in the rat. Israel Jr. Med. Sci. 8, 807.
Fine, J. B. and DiGirolamo, M. (1997) A simple method to predict cellular density in adipocyte metabolic incubations. Int. J. Obes. 21, 764–768.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Humana Press Inc.
About this protocol
Cite this protocol
DiGirolamo, M., Fine, J.B. (2001). Cellularity Measurements. In: Ailhaud, G. (eds) Adipose Tissue Protocols. Methods in Molecular Biology™, vol 155. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-231-7:065
Download citation
DOI: https://doi.org/10.1385/1-59259-231-7:065
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-747-2
Online ISBN: 978-1-59259-231-9
eBook Packages: Springer Protocols