Assessment of White Adipose Tissue Metabolism by Measurement of Arteriovenous Differences
Adipose tissue (AT) is more than a collection of adipocytes. It is a highly organized tissue in which different cell types interact, and in which a complex mix of hormones and substrates arriving in the plasma, together with neural input and the rate of blood flow (BF), all regulate metabolic activity. These multiple, interacting influences cannot be reproduced in vitro, and, hence, if we wish to understand the integration of AT metabolism in the whole body, it is essential to perform studies of AT metabolism in vivo. There are a number of ways in which this can be done (1). One of the most specific and informative, in a quantitative sense, is the measurement of arteriovenous (A-V) differences across the tissue.
KeywordsHydrolysis Catheter Glycerol Lactate Lipase
- 2.Wolfe, R. R. (1984) Tracers in Metabolic Research: Radioisotope and Stable Isotope/Mass Spectrometry Methods. Alan R. Liss, New York.Google Scholar
- 5.Holloway, B. R., Stribling, D., Freeman, S., and Jamieson, L. (1985) Thermogenic role of adipose tissue in the dog. Int. J. Obesity 9, 423–432.Google Scholar
- 6.Gooden, J. M., Campbell, S. L., and van der Walt, J. G. (1986) Measurement of blood flow and lipolysis in the hindquarter tissues of the fat-tailed sheep in vivo. Quart. J. Exp. Physiol. 71, 537–547.Google Scholar
- 8.Frayn, K. N. (1992) Studies of human adipose tissue in vivo, in Energy metabolism: tissue determinants and cellular corollaries (Kinney J. M. and Tucker, H. N., eds.), Raven Press, Philadelphia, pp. 267–295.Google Scholar
- 10.Fielding, B. A., Humphreys, S. M., Shadid, S., and Frayn, K. N. (1995) Arterio-venous differences across human adipose tissue for mono-, di-and tri-acylglycerols before and after a high-fat meal. Endocrinol. Metab. 2, 13–17.Google Scholar