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Glycogen Synthase Activity in Adipose Tissue

Methods for Freeze-Clamping and Assay
  • Heidi K. Ortmeyer
Part of the Methods in Molecular Biology™ book series (MIMB, volume 155)

Abstract

One of the best markers of in vivo insulin-action at insulin sensitive tissues is an increase in glycogen synthase (GS) activity. GS is the rate-limiting enzyme of GS. The activity of glycogen synthase is increased by dephosphorylation, which converts the dependent form (dependent on glucose 6-phosphate [G6P]) to the independent form. GS activity can be increased by an increase in the activities of protein (serine/threonine) phosphatases, which dephosphorylate the enzyme, and/or by a decrease in the activities of protein (serine/threo-nine) kinases, which phosphorylate the enzyme.

Keywords

Adipose Tissue Homogenization Buffer Euglycemic Hyperinsulinemic Clamp Aluminum Block Place Filter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Heidi K. Ortmeyer
    • 1
  1. 1.Obesity and Diabetes Research CenterUniversity of Maryland School of MedicineBaltimoreUSA

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