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In Situ Detection of Epstein-Barr Virus DNA and Viral Gene Products

  • Protocol
Epstein-Barr Virus Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 174))

Abstract

Primary Epstein-Barr virus (EBV) infection is followed by a life-long persistence of the virus in the B-cell compartment of the host (1,2). Small numbers of EBV-carrying B cells have been identified in the peripheral blood as well as in lymphoid and nonlymphoid tissues of chronic virus carriers (3,4). This is relevant to the study of human tumors. The detection of EBV DNA in a tumor by polymerase chain reaction (PCR) usually does not permit conclusions as to whether this is due to the presence of the virus in the tumor cell population or to the presence of EBV-carrying “by-stander” B cells in the tissue. For a meaningful analysis of EBV infection, it is therefore necessary in many instances to establish the cellular location of the virus using morphology-based techniques.

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Author information

Authors and Affiliations

  1. Pathologisches Institut, Friedrich-Alexander Universität, Erlangen, Germany

    Gerald Niedobitek

  2. Gerhard-Domagk-Institut für Pathologie, Universität Münster, Münster, Germany

    Hermann Herbst

Authors
  1. Gerald Niedobitek
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  2. Hermann Herbst
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Editor information

Editors and Affiliations

  1. I.B.L.S. Division of Molecular Genetics, University of Glasgow, UK

    Joanna B. Wilson

  2. I.B.L.S. Division of Biochemistry and Molecular Biology, University of Glasgow, UK

    Gerhard H. W. May

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© 2001 Humana Press Inc., Totowa, NJ

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Niedobitek, G., Herbst, H. (2001). In Situ Detection of Epstein-Barr Virus DNA and Viral Gene Products. In: Wilson, J.B., May, G.H.W. (eds) Epstein-Barr Virus Protocols. Methods in Molecular Biology™, vol 174. Humana Press. https://doi.org/10.1385/1-59259-227-9:79

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  • DOI: https://doi.org/10.1385/1-59259-227-9:79

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-690-1

  • Online ISBN: 978-1-59259-227-2

  • eBook Packages: Springer Protocols

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