Abstract
The epidermis shares many structural similarities to other epithelia throughout the body. All epithelia function as a barrier protecting the internal organs. The epidermis of the skin protects the exterior of the body, whereas other forms of epithelia line the airways, blood vessels, and gastrointestinal, urinary, and reproductive tracts. Some glandular epithelia secret substances such as sweat, mucus, and hormones. All epithelia are avascular and consist of closely packed cells, which are tightly attached to one another via cell junctions. This tight structure allows all epithelia to closely regulate the movement of materials such as ions, nutrients, and secretory products (1,2).
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References
MacKie, R. (1991) Clinical Dermatology. Oxford University Press, Oxford, UK.
Harris, A. (editor) (1996) Epithelial Cell Culture. Cambridge University Press, Cambridge, UK.
Fuchs, E. (1988) Keratins as biochemical markers of epithelial differentiation. Trends Genet. 4, 277–281.
Fuchs, E. (1993) Epidermal differentiation and keratin gene expression. J. Cell Sci. 17, 197–208.
Fuchs, E. and Byrne, C. (1994) The epidermis: rising to the surface. Curr. Opin. Genet. Dev. 4, 725–736.
Baumberger, J. P., Suntzeff, V., and Cowdry, E. V. (1942) Methods for the separation of dermis and some physiological and chemical properties of isolated epidermis. J. Natl. Cancer Inst. 2, 413–423.
Marrs, J. M. and Voorhees, J. J. (1971) A method for the bioassay of an epidermal chalone-like inhibitor. J. Invest. Dermatol. 56, 174–181.
Skerrow, C. J. and Skerrow, D. (1985) A survey of methods for the isolation and fractionation of epidermal tissue and cells, in Methods in Skin Research (Skerrow, D. and Skerrow, C. J., eds.), Wiley, New York, NY, pp. 609–650.
Wilson, J. B., Weinberg, W., Johnson, R., Yuspa, S., and Levine, A. (1990) Expression of the BNLF-1 oncogene of Epstein-Barr virus in the skin of transgenic mice induces hyperplasia and aberrant expression of Keratin 6. Cell 61, 1315–1327.
Yuspa, S. H., Hawley-Nelson, P., Stanley, J. R., and Hennings, H. (1980) Epidermal cell culture. Transplant.Proc. 12, 114–122
Hennings, H., Michael, D., Cheng, C., Steinert, P., Holbrook, K., and Yuspa, S. H. (1980) Calcium regulation of growth and differentiation of mouse epidermal cells in culture. Cell 19, 245–254.
Slaga, T. J., Thomson, S., and Schwarz, J. A. (1977) Binding of dexamethasone by the subcellular fractions of mouse epidermis and dermis. J. Invest. Dermatol. 68, 307–309.
Parkinson, E. K. and Yeudall, W. A. (1992) The epidermis, in Culture of Epithelial Cells (Freshney, R. I., (ed.), Wiley-Liss, Inc., Chichester, NY, pp. 59–80.
Green, H. (1977) Terminal differentiation of cultured human epidermal cells. Cell 11, 405–416.
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© 2001 Humana Press Inc., Totowa, NJ
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Macdiarmid, J., Wilson, J.B. (2001). Separation of Epidermal Tissue from Underlying Dermis and Primary Keratinocyte Culture. In: Wilson, J.B., May, G.H.W. (eds) Epstein-Barr Virus Protocols. Methods in Molecular Biology™, vol 174. Humana Press. https://doi.org/10.1385/1-59259-227-9:401
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DOI: https://doi.org/10.1385/1-59259-227-9:401
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