Abstract
The Epstein-Barr viral nuclear antigen 2 (EBNA2) plays a key role during establishment and maintenance of B-cell immortalization after Epstein-Barr virus (EBV) infection (1). EBNA2 acts as a transcriptional activator of cellular and viral genes (2–8) and is tethered to EBNA2 responsive promoter elements by interaction with a cellular DNA binding protein (9,10). We purified this transcription factor by classical chromatographic methods, however, instead of the usual final DNA affinity column step, we applied a magnetic DNA affinity purification protocol. This increased the purity of the transcription factor from about 0.01% to almost homogeneity and allowed subsequent identification of the protein as RBP-Jκ (11).
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References
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© 2001 Humana Press Inc., Totowa, NJ
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Strobl, L.J., Zimber-Strobl, U. (2001). Magnetic DNA Affinity Purification of a Cellular Transcription Factor. In: Wilson, J.B., May, G.H.W. (eds) Epstein-Barr Virus Protocols. Methods in Molecular Biology™, vol 174. Humana Press. https://doi.org/10.1385/1-59259-227-9:271
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DOI: https://doi.org/10.1385/1-59259-227-9:271
Publisher Name: Humana Press
Print ISBN: 978-0-89603-690-1
Online ISBN: 978-1-59259-227-2
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