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Genetic Analysis and Gene Expression with Mini-Epstein-Barr Virus Plasmids

  • Protocol
Epstein-Barr Virus Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 174))

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Abstract

Upon infection with Epstein-Barr virus (EBV), primary human B-lymphocytes are efficiently immortalized and give rise to lymphoblastoid cell lines in vitro. Four of the 11 viral genes expressed in the immortalized B cells have been found to be essential genetically for the process of immortalization: the EBV nuclear antigens EBNA2, EBNA3a, and EBNA3c, and the latent membrane protein 1 (LMP1) (1–5). Since EBNA1 maintains the status of the EBV genomes in the proliferating B cells, it might also be indispensable (6).

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Author information

Authors and Affiliations

  1. Boehringer Ingelheim Pharma KG, Ingelheim, Germany

    Ellen Kilger

  2. Institut für Klinische Molekularbiologie und Tumorgenetik, Abteilung Genvektoren, GSF, München, Germany

    Wolfgang Hammerschmidt

Authors
  1. Ellen Kilger
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  2. Wolfgang Hammerschmidt
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Editor information

Editors and Affiliations

  1. I.B.L.S. Division of Molecular Genetics, University of Glasgow, UK

    Joanna B. Wilson

  2. I.B.L.S. Division of Biochemistry and Molecular Biology, University of Glasgow, UK

    Gerhard H. W. May

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© 2001 Humana Press Inc., Totowa, NJ

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Kilger, E., Hammerschmidt, W. (2001). Genetic Analysis and Gene Expression with Mini-Epstein-Barr Virus Plasmids. In: Wilson, J.B., May, G.H.W. (eds) Epstein-Barr Virus Protocols. Methods in Molecular Biology™, vol 174. Humana Press. https://doi.org/10.1385/1-59259-227-9:23

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  • DOI: https://doi.org/10.1385/1-59259-227-9:23

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-690-1

  • Online ISBN: 978-1-59259-227-2

  • eBook Packages: Springer Protocols

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