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In Vitro Assays to Study Epithelial Cell Differentiation

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Epstein-Barr Virus Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 174))

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Abstract

The development of in vitro systems for the culture of human epithelial cells has aided the study of epithelial cell transformation by the epitheliotrophic papilloma-viruses (1). Although long-term proliferation of keratinocytes in vitro can now be achieved, the tissue-culture environment is not optimal for differentiation, hampering the study of virus:cell interactions in differentiating epithelial cells.

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References

  1. Chow, L. T. and Broker, T. R. (1997) In vitro experimental systems for HPV: epithelial raft cultures for investigations of viral reproduction and pathogenesis and for genetic analyses of viral proteins and regulatory sequences. Clin. Dermatol. 15, 217–227.

    Article  PubMed  CAS  Google Scholar 

  2. Watt, F. M. (1983) Involucrin and other markers of keratinocyte terminal differentiation. J. Investig. Dermatolo. 81, S100–S103.

    Article  Google Scholar 

  3. Asselineau, D., Bernhard, B., Bailly, C., and Darmon, M. (1985) Epidermal morphogenesis and induction of the 67-kd keratin polypeptide by culture of human keratinocytes at the liquid-air interface. Exp. Cell Res. 159, 536–539.

    Article  PubMed  CAS  Google Scholar 

  4. Kopan, R., Traska, G., and Fuchs, E. (1987) Retinoids as important regulators of terminal differentiation-examining keratin expression in individual epidermal-cells at various stages of keratinization. J. Cell Biol. 105, 427–440.

    Article  PubMed  CAS  Google Scholar 

  5. Rheinwald, J. G. and Beckett, M. A. (1981) Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultured from Human squamous-cell carcinomas. Cancer Res. 41, 1657–1663.

    PubMed  CAS  Google Scholar 

  6. Parkinson, E. K., Grabham, P., and Emmerson, A. (1983) A subpopulation of cultured human keratinocytes which is resistant to the induction of terminal differentiation-related changes by phorbol, 12-myristate, 13-acetate—evidence for an increase in the resistant population following transformation. Carcinogenesis 4, 857–861.

    Article  PubMed  CAS  Google Scholar 

  7. Ryle, C. M., Breitkreutz, D., Stark, H. J., Leigh, I. M., Steinert, P. M., Roop, D., and Fusenig, N. E. (1989) Density-dependent modulation of synthesis of keratin-1 and keratin-10 in the Human keratinocyte line HaCat and in ras-transfected tumorigenic clones. Differentiation 40, 42–54.

    Article  PubMed  CAS  Google Scholar 

  8. Fuchs, E. (1990) Epidermal differentiation: the bare essentials. J. Cell Biol. 111, 2807–2814.

    Article  PubMed  CAS  Google Scholar 

  9. Green, H. (1977) Terminal differentiation of cultured Human epidermal cells. Cell 11, 405–416.

    Article  PubMed  CAS  Google Scholar 

  10. Rubin, A. L. and Rice, R. H. (1986) Differential regulation by retinoic acid and calcium of transglutaminases in cultured neoplastic and normal human keratinocytes. Cancer Res. 46, 2356–2361.

    PubMed  CAS  Google Scholar 

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© 2001 Humana Press Inc., Totowa, NJ

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Dawson, C.W., Young, L.S. (2001). In Vitro Assays to Study Epithelial Cell Differentiation. In: Wilson, J.B., May, G.H.W. (eds) Epstein-Barr Virus Protocols. Methods in Molecular Biology™, vol 174. Humana Press. https://doi.org/10.1385/1-59259-227-9:173

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  • DOI: https://doi.org/10.1385/1-59259-227-9:173

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-690-1

  • Online ISBN: 978-1-59259-227-2

  • eBook Packages: Springer Protocols

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