Abstract
The quantitative, competitive polymerase chain reaction (PCR) assay outlined in this chapter was designed for the detection and quantitation of replicated DNAs in both short-term and long-term assays (1). Quantitative, competitive PCR can be used to study both the contribution of proteins to the replication of oriP-based plasmids (1) as well as the requirements for specific DNA sequences to support replication of a plasmid (2). Advantages of this assay include an increased sensitivity and a decreased time required to analyze samples relative to DNA blots, the traditional assay used to study replication of oriP-containing plasmids in the presence of EBNA-1 (3–8).
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© 2001 Humana Press Inc., Totowa, NJ
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Kirchmaier, A.L. (2001). Analysis of Replication of oriP-Based Plasmids by Quantitative, Competitive PCR. In: Wilson, J.B., May, G.H.W. (eds) Epstein-Barr Virus Protocols. Methods in Molecular Biology™, vol 174. Humana Press. https://doi.org/10.1385/1-59259-227-9:13
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DOI: https://doi.org/10.1385/1-59259-227-9:13
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